Yang Zhongguang, Kou Songzi, Wei Xi, Zhang Fengjie, Li Fei, Wang Xiao-Wei, Lin Yuan, Wan Chao, Zhang Wen-Bin, Sun Fei
Department of Chemical and Biological Engineering and Center of Systems Biology & Human Health, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.
Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen 518036, China.
ACS Macro Lett. 2018 Dec 18;7(12):1468-1474. doi: 10.1021/acsmacrolett.8b00845. Epub 2018 Nov 28.
We report the synthesis of a series of elastin-like polypeptide (ELP)-based molecular networks through the combined use of the covalent bond-forming SpyTag/SpyCatcher chemistry, physically entangled p53dim domains (Xs), and site-directed mutagenesis. The resulting networks shared similar chemical composition but differed significantly in their viscoelasticity. These materials exhibited excellent compatibility toward encapsulated fibroblasts and stem cells. These results point to a versatile strategy for designing viscoelastic materials by tapping into diverse protein-protein interactions.
我们报告了通过共价键形成的SpyTag/SpyCatcher化学、物理缠结的p53dim结构域（Xs）和定点诱变的联合使用，合成了一系列基于弹性蛋白样多肽（ELP）的分子网络。所得网络具有相似的化学成分，但粘弹性差异显著。这些材料对封装的成纤维细胞和干细胞表现出优异的相容性。这些结果表明了一种通过利用多种蛋白质-蛋白质相互作用来设计粘弹性材料的通用策略。
