Meng Tiantian, Deng Juying, Xiao Dingfu, Arowolo Muhammed Adebayo, Liu Chunming, Chen Liang, Deng Wei, He Shaoping, He Jianhua
College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
Institute of Animal Husbandry and Aquaculture, Huaihua Academy of Agricultural Sciences, Huaihua, China.
Front Nutr. 2022 May 16;9:821272. doi: 10.3389/fnut.2022.821272. eCollection 2022.
Resveratrol (RSV) is a natural polyphenolic compound with potent antioxidant and anti-inflammatory properties. This study aimed to investigate the protective effects of RSV supplementation on the inflammatory responses of broilers during heat stress. A total of 432 28-d-old white-feathered broilers (817 crossbred chicken) with an average weight of 549 ± 4 g were randomly allotted to 4 equal groups (Half male and half female, 6 replicates/group, 18 chickens/replicate), including normal temperature (NT) group (24 ± 2°C for 24 h/d, basal diet), NT+RSV group (24 ± 2°C for 24 h/d, basal diet + RSV), heat stress (HT) group (37 ± 2°C for 8 h/d, basal diet), and HT+RSV group (37 ± 2°C for 8 h/d, basal diet + RSV). Serum samples were collected on d 7 and 14 of heat stress, and thymus, spleen, jejunum, and bursa of Fabricius samples were collected and analyzed on d14. RSV treatment decreased the feed conversion ratio, partially reversed the negative alternations in body weight, average daily gain, and average daily feed intake caused by heat stress. RSV treatment also decreased the elevated levels of corticosterone on d 14, adrenocorticotropic hormone, and triiodothyronine in serum on d 7 caused by heat stress, and significantly increased the villus height to crypt depth ratio in the jejunum on d 14. Dietary RSV also reduced heat stress-induced splenic pro-inflammatory cytokine concentrations. TUNEL assay showed that RSV significantly reduced heat stress-induced the number of apoptotic cells. Remarkably, RSV down-regulated some splenic related genes for apoptosis genes, including , and mRNA levels induced by heat stress. According to GO and KEGG enrichment analyses, the differential genes between HT and HT + RSV groups were mainly associated with immune system process, hematopoietic or lymphoid organ development, and toll-like receptor signaling pathway. The relative mRNA expression of κ, heat shock protein 70 (), and were markedly decreased by the combination of RSV and heat stress. These findings showed that RSV might reduce the splenic inflammatory response in heat-stressed white-feather broilers by inhibiting heat stress-induced activation of , and , as well as inhibiting the activation of mitochondrial apoptotic pathways.
白藜芦醇（RSV）是一种具有强大抗氧化和抗炎特性的天然多酚化合物。本研究旨在探讨补充RSV对热应激期间肉鸡炎症反应的保护作用。将432只28日龄平均体重为549±4克的白羽肉鸡（817杂交鸡）随机分为4组（雌雄各半，每组6个重复，每个重复18只鸡），包括常温（NT）组（24±2°C，每天24小时，基础日粮）、NT+RSV组（24±2°C，每天24小时，基础日粮+RSV）、热应激（HT）组（37±2°C，每天8小时，基础日粮）和HT+RSV组（37±2°C，每天8小时，基础日粮+RSV）。在热应激的第7天和第14天采集血清样本，并在第14天采集胸腺、脾脏、空肠和法氏囊样本进行分析。RSV处理降低了饲料转化率，部分逆转了热应激引起的体重、平均日增重和平均日采食量的负面变化。RSV处理还降低了热应激引起的第14天血清中皮质酮、促肾上腺皮质激素水平升高以及第7天血清中三碘甲状腺原氨酸水平升高，并显著提高了第14天空肠绒毛高度与隐窝深度的比值。日粮RSV还降低了热应激诱导的脾脏促炎细胞因子浓度。TUNEL检测显示，RSV显著减少了热应激诱导的凋亡细胞数量。值得注意的是，RSV下调了一些脾脏相关凋亡基因，包括热应激诱导的 、 和 mRNA水平。根据GO和KEGG富集分析，HT组和HT+RSV组之间的差异基因主要与免疫系统过程、造血或淋巴器官发育以及Toll样受体信号通路有关。RSV与热应激联合作用显著降低了κ、热休克蛋白70（ ）和 的相对mRNA表达。这些发现表明，RSV可能通过抑制热应激诱导的 、 和 的激活以及抑制线粒体凋亡途径的激活来减轻热应激白羽肉鸡的脾脏炎症反应。
