Lork Alicia A, Vo Kim L L, Phan Nhu T N
Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
Front Synaptic Neurosci. 2022 May 16;14:854957. doi: 10.3389/fnsyn.2022.854957. eCollection 2022.
A nerve cell is a unit of neuronal communication in the nervous system and is a heterogeneous molecular structure, which is highly mediated to accommodate cellular functions. Understanding the complex regulatory mechanisms of neural communication at the single cell level requires analytical techniques with high sensitivity, specificity, and spatial resolution. Challenging technologies for chemical imaging and analysis of nerve cells will be described in this review. Secondary ion mass spectrometry (SIMS) allows for non-targeted and targeted molecular imaging of nerve cells and synapses at subcellular resolution. Cellular electrochemistry is well-suited for quantifying the amount of reactive chemicals released from living nerve cells. These techniques will also be discussed regarding multimodal imaging approaches that have recently been shown to be advantageous for the understanding of structural and functional relationships in the nervous system. This review aims to provide an insight into the strengths, limitations, and potentials of these technologies for synaptic and neuronal analyses.
神经细胞是神经系统中神经元通讯的基本单位，是一种异质分子结构，高度介导以适应细胞功能。在单细胞水平上理解神经通讯的复杂调控机制需要具有高灵敏度、特异性和空间分辨率的分析技术。本文将介绍用于神经细胞化学成像和分析的具有挑战性的技术。二次离子质谱（SIMS）能够以亚细胞分辨率对神经细胞和突触进行非靶向和靶向分子成像。细胞电化学非常适合定量从活神经细胞释放的反应性化学物质的量。还将讨论这些技术在多模态成像方法方面的应用，最近的研究表明这些方法对于理解神经系统中的结构和功能关系具有优势。本综述旨在深入了解这些技术在突触和神经元分析方面的优势、局限性和潜力。
