Department of Chemistry, Yanbian University, Park Road 977, Yanji 133002, Jilin, China.
Interdisciplinary Program of Biological Functional Molecules, College of Integration Science, Yanbian University, Park Road 977, Yanji 133002, Jilin, China.
Anal Chem. 2022 Jun 14;94(23):8474-8482. doi: 10.1021/acs.analchem.2c01313. Epub 2022 Jun 2.
A circular nonuniform electric field strategy coupled with gel electrophoresis was proposed to control the precise separation and efficient concentration of nano- and microparticles. The circular nonuniform electric field has the feature of exponential increase in the electric field intensity along the radius, working with three functional zones of migration, acceleration, and concentration. The distribution form of electric field lines is regulated in functional zones to control the migration behaviors of particles for separation and concentration by altering the relative position of the ring electrode (outside) and rodlike electrode (inner). The circular nonuniform electric field promotes the target-type and high-precision separation of nanoparticles based on the difference in charge-to-size ratio. The concentration multiple of nanoparticles is also controlled randomly with the alternation of radius, taking advantage of vertical extrusion and concentric converging of the migration path. This work provides a brand new insight into the simultaneous separation and concentration of particles and is promising for developing a versatile tool for the separation and preparation of various samples instead of conventional methods.
一种环形非均匀电场策略与凝胶电泳相结合，被提出用于控制纳米和微米颗粒的精确分离和高效浓缩。环形非均匀电场具有沿半径方向电场强度呈指数增加的特点，与迁移、加速和浓缩三个功能区一起工作。通过改变环电极（外部）和棒状电极（内部）的相对位置，可以调节功能区中的电场线分布形式，控制颗粒的迁移行为，从而实现分离和浓缩。环形非均匀电场基于电荷-尺寸比的差异，促进了基于目标型和高精度的纳米颗粒分离。通过交替改变半径，纳米颗粒的浓缩倍数也可以随机控制，利用迁移路径的垂直挤压和同心收敛。这项工作为颗粒的同时分离和浓缩提供了全新的视角，有望开发出一种通用的工具，用于各种样品的分离和制备，而不是传统的方法。
