Liu Lu, Cui Jiaxuan, Chen Peng, Fatima Zakia, Xing Yuhang, Liu Huwei, Ren Xiangshan, Li Donghao
Department of Pathology and Key Laboratory of Pathobiology, State Ethnic Affairs Commission, Medical College, Yanbian University, Park Road 977, Yanji, Jilin Province 133002, China; Department of Chemistry, Yanbian University, Park Road 977, Yanji, Jilin Province 133002, China; Key Laboratory of Agrifood Quality and Safety Evaluation, Yanbian University, Yanji, Jilin Province 133002, China.
Department of Chemistry, Yanbian University, Park Road 977, Yanji, Jilin Province 133002, China.
J Chromatogr A. 2024 Jul 19;1727:464990. doi: 10.1016/j.chroma.2024.464990. Epub 2024 May 10.
An approach for the controllable separation and concentration of nucleic acid using a circular nonuniform electric field was proposed and developed. Using six different lengths of DNA molecules as standard samples, the distribution of the gradient electric field was increased from the outer circular electrode to the inner rod-shaped electrode, contributing to the migration of DNA molecules at a velocity gradient towards the region with the strongest inner electric field. The DNA molecules were arranged in a distribution of concentric circles that aligned with the distribution of concentric equipotential lines. The concentration of DNA multiplied with the alternation of radius. As a result, this platform allowed simultaneous DNA separation, achieving a resolution range of 1.17-3.03 through an extended electrophoresis time, resulting in enhanced concentration factors of 1.08-6.27. Moreover, the manipulation of the relative height of the inner and outer electrodes enabled precise control over the distribution and the deflection degree of electric field lines, leading to accurate control over DNA deflection.
提出并开发了一种利用圆形非均匀电场对核酸进行可控分离和浓缩的方法。以六种不同长度的DNA分子作为标准样品，梯度电场的分布从外圆形电极向内部棒状电极增加，促使DNA分子以速度梯度向内部电场最强的区域迁移。DNA分子排列成与同心等势线分布对齐的同心圆分布。DNA的浓度随着半径的变化而增加。结果，该平台允许同时进行DNA分离，通过延长电泳时间实现了1.17 - 3.03的分辨率范围，从而使浓缩因子提高到1.08 - 6.27。此外，通过操纵内、外电极的相对高度，可以精确控制电场线的分布和偏转程度，从而实现对DNA偏转的精确控制。
