Key Laboratory of Biomass Chemical Engineering of Ministry of Education and Zhejiang Key Laboratory of Smart Biomaterials, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China.
Angew Chem Int Ed Engl. 2022 Sep 5;61(36):e202202128. doi: 10.1002/anie.202202128. Epub 2022 Jul 27.
Fluorescent imaging with fluorophores has become a powerful way to explore complex biological systems and visualize nanoparticles for drug delivery. However, it is challenging to develop fluorophores with ideal physical and optical properties. We report a method to synthesize cyanine nanodots with a single-molecule structure, well-defined particle size, customizable fluorescent spectrum, and bright and stable fluorescence. These cyanine nanodots are acquired by the divergent synthesis of cyanine-dye-cored polylysine (PLL) dendrimers. We demonstrated the feasibility of the method by synthesizing cyanine 3 (Cy3), cyanine 5 (Cy5), or cyanine 7 (Cy7) cored single-molecule nanodots up to eight generations with a size of around 11 nm. We show that these cyanine nanodots are capable of multiple biomedical applications, including multicolor cellular tracing and cancer imaging. These cyanine nanodots possess many merits of organic dots and quantum dots that are promising for future application.
荧光团的荧光成像已成为探索复杂生物系统和可视化纳米粒子用于药物输送的强大方法。然而，开发具有理想物理和光学性质的荧光团具有挑战性。我们报告了一种用单分子结构、确定的粒径、可定制的荧光光谱、明亮稳定的荧光合成菁染料纳米点的方法。这些菁纳米点是通过发散合成菁染料核聚赖氨酸（PLL）树状大分子获得的。我们通过合成多达 8 代的大小约为 11nm 的菁 3（Cy3）、菁 5（Cy5）或菁 7（Cy7）核单分子纳米点，证明了该方法的可行性。我们表明，这些菁纳米点能够进行多种生物医学应用，包括多色细胞示踪和癌症成像。这些菁纳米点具有有机点和量子点的许多优点，有望在未来得到应用。
