Cong Hailin, Wang Kaiqi, Zhou Zhuha, Yang Jiajia, Piao Ying, Yu Bing, Shen Youqing, Zhou Zhuxian
Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, State Key Laboratory of Bio-Fibers and Eco-Textiles, Qingdao University, Qingdao 266071, China.
Key Laboratory of Biomass Chemical Engineering of Ministry of Education and Center for Bionanoengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
ACS Nano. 2020 May 26;14(5):5887-5900. doi: 10.1021/acsnano.0c01034. Epub 2020 May 7.
Specific labeling of biomarkers with bright and high photostable fluorophores is vital in fluorescent imaging applications. Here, we report a general strategy to develop single-molecule dendritic nanodots with finely tunable optical properties for fluorescent imaging. The well-defined nanodots are based on the divergent growth of biodegradable polylysine dendrimers with a fluorophore as the core. By tuning the size and surface chemistry, we obtained fluorescent nanodots with excellent brightness and photostability, favorable pharmacokinetics, and multivalent tumor-targeting capability. The nanodots provided robust, stable, long-lasting, and specific fluorescence enhancement in tumor tissue with an tumor-to-normal ratio (TNR) of ∼3 and lasting over 5 days and an TNR up to ∼17, holding considerable promise for cancer imaging and image-guided surgery. This strategy significantly improves the performance of fluorophores and can be applied to other modality imaging probes.
使用明亮且高光稳定性的荧光团对生物标志物进行特异性标记在荧光成像应用中至关重要。在此，我们报告了一种开发具有精细可调光学特性的单分子树枝状纳米点用于荧光成像的通用策略。这些定义明确的纳米点基于以荧光团为核心的可生物降解聚赖氨酸树枝状大分子的发散生长。通过调整尺寸和表面化学性质，我们获得了具有出色亮度和光稳定性、良好药代动力学以及多价肿瘤靶向能力的荧光纳米点。这些纳米点在肿瘤组织中提供了强大、稳定、持久且特异性的荧光增强，肿瘤与正常组织的比率（TNR）约为3，持续超过5天，TNR高达约17，在癌症成像和图像引导手术方面具有巨大潜力。该策略显著提高了荧光团的性能，并且可应用于其他模态成像探针。
