Liu Songlu, Shang Wenting, Song Jian, Li Qiubai, Wang Liang
Department of Radiology, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, Beijing, 100190, China.
J Nanobiotechnology. 2024 Jul 17;22(1):421. doi: 10.1186/s12951-024-02704-0.
Prostate cancer (PCa) is the most prevalent cancer among males, emphasizing the critical need for precise diagnosis and treatment to enhance patient prognosis. Recent studies have extensively utilized urine exosomes from patients with cancer for targeted delivery. This study aimed to employ highly sensitive magnetic particle imaging (MPI) and fluorescence molecular imaging (FMI) to monitor the targeted delivery of an exosome-loaded platform at the tumour site, offering insights into a potential combined photothermal and magnetic thermal therapy regime for PCa.
MPI and FMI were utilized to monitor the in vivo retention performance of exosomes in a prostate tumour mouse model. The exosome-loaded platform exhibited robust homologous targeting ability during imaging (SPIONs@EXO-Dye:66·48%±3·85%; Dye-SPIONs: 34·57%±7·55%, **P<0·01), as verified by in vitro imaging and in vitro tissue Prussian blue staining.
The experimental data underscore the feasibility of using MPI for in vivo PCa imaging. Furthermore, the exosome-loaded platform may contribute to the precise diagnosis and treatment of PCa.
前列腺癌（PCa）是男性中最常见的癌症，这凸显了精确诊断和治疗以改善患者预后的迫切需求。最近的研究广泛利用癌症患者的尿液外泌体进行靶向递送。本研究旨在采用高灵敏度磁粒子成像（MPI）和荧光分子成像（FMI）来监测负载外泌体的平台在肿瘤部位的靶向递送情况，为前列腺癌潜在的光热和磁热联合治疗方案提供见解。
利用MPI和FMI监测外泌体在前列腺肿瘤小鼠模型中的体内滞留性能。负载外泌体的平台在成像过程中表现出强大的同源靶向能力（超顺磁性氧化铁纳米粒子@外泌体 - 染料：66.48%±3.85%；染料 - 超顺磁性氧化铁纳米粒子：34.57%±7.55%，**P<0.01），体外成像和体外组织普鲁士蓝染色验证了这一点。
实验数据强调了使用MPI进行前列腺癌体内成像的可行性。此外，负载外泌体的平台可能有助于前列腺癌的精确诊断和治疗。
