The State Key Laboratory of Membrane Biology; Tsinghua-Peking Joint Center for Life Sciences; Beijing Frontier Research Center for Biological Structures; School of Life Science, Tsinghua University, Beijing, China.
Ministry of Education Key Laboratory of Protein Sciences; Tsinghua-Peking Joint Center for Life Sciences; Beijing Advanced Innovation Center for Structural Biology; Beijing Frontier Research Center for Biological Structures; School of Life Science, Tsinghua University, Beijing, China.
Nat Cell Biol. 2022 Jun;24(6):825-832. doi: 10.1038/s41556-022-00920-0. Epub 2022 Jun 2.
Various mechanisms contribute to membrane repair but the machinery that mediates the repair of large wounds on the plasma membrane is less clear. We found that shortly after membrane damage, Tetraspanin-enriched macrodomains are assembled around the damage site. Tetraspanin-enriched macrodomains are in the liquid-ordered phase and form a rigid ring around the damaged site. This restricts the spread of the damage and prevents membrane disintegration, thus facilitating membrane repair by other mechanisms. Functionally, Tetraspanin 4 helps cells mitigate damage caused by laser, detergent, pyroptosis and natural killer cells. We propose that assembly of Tetraspanin-enriched macrodomains creates a physical barrier to contain membrane damage.
多种机制有助于修复细胞膜，但介导质膜大伤口修复的机制尚不清楚。我们发现，在细胞膜损伤后不久，富含四跨膜蛋白的巨域就会围绕损伤部位组装。富含四跨膜蛋白的巨域处于液晶有序相，并在损伤部位周围形成刚性环。这限制了损伤的扩散，防止了膜的崩解，从而促进了其他机制的膜修复。功能上，四跨膜蛋白 4 有助于细胞减轻激光、去污剂、细胞焦亡和自然杀伤细胞造成的损伤。我们提出，富含四跨膜蛋白的巨域的组装形成了一个物理屏障，以容纳膜损伤。
