Agricultural Botany Department, Faculty of Agriculture, Saba Basha, Alexandria University, Alexandria, 21531, Egypt.
Bioinformatics Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Sadat City, Egypt.
Mol Biol Rep. 2022 Jun;49(6):5645-5657. doi: 10.1007/s11033-022-07574-z. Epub 2022 Jun 2.
DNA barcoding have been considered as a tool to facilitate species identification based on its simplicity and high-level accuracy in compression to the complexity and subjective biases linked to morphological identification of taxa. MaturaseK gene (MatK gene) of the chloroplast is very vital in the plant system which is involved in the group II intron splicing. The main objective of this study is to determine the relative utility of the "MatK" chloroplast gene for barcoding in 15 legume as a tool to facilitate species identification based on their simplicity and high-level accuracy linked to morphological identification of taxa.
MatK gene sequences were submitted to GenBank and the accession numbers were obtained with sequence length ranging from 730 to 1545 nucleotides. These DNA sequences were aligned with database sequence using PROMALS server, Clustal Omega server and Bioedit program. Maximum likelihood and neighbor-joining algorithms were employed for constructing phylogeny. Overall, these results indicated that the phylogenetic tree analysis and the evolutionary distances of an individual dataset of each species were agreed with a phylogenetic tree of all each other consisting of two clades, the first clade comprising (Enterolobium contortisiliquum, Albizia lebbek), Acacia saligna, Leucaena leucocephala, Dichrostachys Cinerea, (Delonix regia, Parkinsonia aculeata), (Senna surattensis, Cassia fistula, Cassia javanica) and Schotia brachypetala were more closely to each other, respectively. The remaining four species of Erythrina humeana, (Sophora secundiflora, Dalbergia Sissoo, Tipuana Tipu) constituted the second clade.
Moreover, their sequences could be successfully utilized in single nucleotide polymorphism or as part of the sequence as DNA fragment analysis utilizing polymerase chain reaction in plant systematic. Therefore, MatK gene is considered promising a candidate for DNA barcoding in the plant family Fabaceae and provides a clear relationship between the families.
DNA 条形码已被认为是一种基于其简单性和对分类群形态鉴定的高准确性来促进物种鉴定的工具，因为它可以压缩分类群形态鉴定的复杂性和主观性偏见。叶绿体的 maturaseK 基因（MatK 基因）在植物系统中非常重要，它参与了组 II 内含子的剪接。本研究的主要目的是确定“MatK”叶绿体基因在 15 种豆科植物中的相对实用性，作为一种基于其简单性和对分类群形态鉴定的高准确性来促进物种鉴定的工具。
MatK 基因序列被提交到 GenBank 并获得了长度从 730 到 1545 个核苷酸的序列注册号。这些 DNA 序列使用 PROMALS 服务器、Clustal Omega 服务器和 Bioedit 程序与数据库序列进行比对。使用最大似然法和邻接法构建系统发育树。总体而言，这些结果表明，每个物种的个体数据集的系统发育树分析和进化距离与由两个分支组成的所有其他物种的系统发育树一致，第一个分支包括（扭叶苏木、勒氏相思）、银荆、银叶金合欢、黄槐（无忧花、使君子）、（海檬果、腊肠树、阿拉伯胶树）和肖黄栌，彼此之间更为接近。其余四个物种（印度黄檀、美丽决明、印度紫檀）构成了第二个分支。
此外，它们的序列可以成功地用于单核苷酸多态性或作为 DNA 片段分析的序列的一部分，利用聚合酶链反应在植物系统中。因此，MatK 基因被认为是植物科 Fabaceae 中 DNA 条形码的有前途的候选者，并提供了家族之间的明确关系。
