Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Brazil.
School of Veterinary, State University of Ceará, Fortaleza, Brazil.
Biofouling. 2022 Apr;38(4):401-413. doi: 10.1080/08927014.2022.2084612. Epub 2022 Jun 2.
is the most important agent of persistent apical periodontitis, and recently, has also been implicated in periapical infections. This study aimed to optimize an and dual-species biofilm protocol for endodontic research. Different physicochemical conditions for biofilm formation were tested. Susceptibility assays to antimicrobials, biochemical composition and an ultra-morphological structure analyses were performed. Reproducible dual-species biofilms were established in BHI medium at 35 °C, for 48 h and in a microaerophilic atmosphere. An increase in biomass and chitin content was detected after vancomycin treatment. Structural analysis revealed that the dual-species biofilm was formed by both microorganisms adhered to the substrate. The proposed protocol could be useful for the study of interkingdom relationships and help to find new strategies against periapical infections.
是持续性根尖周炎最重要的病原体，最近也被认为与根尖周感染有关。本研究旨在优化一种和双菌种生物膜方案用于牙髓学研究。测试了不同的生物膜形成理化条件。进行了抗微生物药物敏感性测定、生物化学组成和超形态结构分析。在 35°C 下，在微需氧环境中，在 BHI 培养基中培养 48 小时，成功建立了可重复的双菌种生物膜。万古霉素处理后检测到生物量和几丁质含量增加。结构分析表明，双菌种生物膜是由附着在基质上的两种微生物形成的。该方案可用于研究种间关系，并有助于寻找针对根尖周感染的新策略。
