State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, China.
Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Biofouling. 2021 Oct-Nov;37(9-10):964-974. doi: 10.1080/08927014.2021.1993836. Epub 2021 Nov 28.
The present study utilized an dual-species biofilm model and an rat post-treatment endodontic disease (PTED) model to investigate whether co-infection of and would aggravate periapical lesions. The results showed that co-culturing yielded a thicker and denser biofilm more tolerant to detrimental stresses compared with the mono-species biofilm, such as a starvation-alkalinity environment, mechanical shear force and bactericidal chemicals. Consistently, co-inoculation of and significantly increased the extent of periapical lesions compared with mono-species infection. Specifically, coexistence of both microorganisms increased osteoclastic bone resorption and suppressed osteoblastic bone formation. The synergistic effects also up-regulated inflammatory cytokines including and . In summary, coexistence of and increased periapical lesions by enhanced biofilm virulence.
本研究采用双物种生物膜模型和大鼠牙髓治疗后疾病（PTED）模型，探讨了 和 是否会加重根尖周病变。结果表明，与单物种生物膜相比，共培养产生的生物膜更厚、更密集，对不利应激（如饥饿-碱性环境、机械剪切力和杀菌化学物质）更耐受。一致的是， 和 的共同接种显著增加了根尖周病变的程度，与单物种感染相比。具体而言，两种微生物的共存增加了破骨细胞的骨吸收并抑制了成骨细胞的骨形成。协同作用还上调了包括 和 在内的炎症细胞因子。总之， 通过增强生物膜毒力， 和 的共存增加了根尖周病变。
