Mahmoudzadeh Elaheh, Nazemiyeh Hossein, Valizadeh Hadi, Khaleseh Farnaz, Mohammadi Samin, Hamedeyazdan Sanaz
Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Iran J Basic Med Sci. 2022 Mar;25(3):364-371. doi: 10.22038/IJBMS.2022.62032.13760.
The current study's objectives were to obtain different extracts and essential oils of and and to determine the chemical composition, as well as to evaluate free radical scavenging activity (IC) and minimum bactericidal concentration (MBC), and the effect of liposomal formulation on antimicrobial properties.
Air-dried powdered aerial parts of and were used. The antioxidant and antibacterial properties, essential oil compositions, total phenol, and flavonoid contents of different fractions were determined by DPPH test, disk diffusion assay, gas chromatography-mass spectrometry, Folin-ciocalteu reagent, and colorimetric assay method, respectively. The film hydration method was used to fabricate nanoparticles.
GC-MS analysis indicated that hexafarnesyl acetone was a major essential oil component. n-butanol and ethyl acetate extracts of had the highest anti-oxidant activity. Extracts of both plants showed antimicrobial activity. The extracts' maximum inhibition zones against were established. A particle size analyzer detected the formulation size of 140 nm. The optimum formulation of liposomes contains the ratio of 75 mg lecithin, 25 mg cholesterol, and 50 mg herbal extract. Despite the nanoparticles' appropriate particle size, the liposomal extract's antimicrobial effect was lower than that of the free form.
Our findings demonstrated that extracts have significant antibacterial and anti-oxidant activities, attributed to their bioactive constituents.
本研究的目的是获取[植物名称1]和[植物名称2]的不同提取物和精油，确定其化学成分，评估自由基清除活性（IC）和最低杀菌浓度（MBC），以及脂质体制剂对抗菌性能的影响。
使用[植物名称1]和[植物名称2]风干的地上部分粉末。分别通过DPPH试验、纸片扩散法、气相色谱 - 质谱联用、福林 - 西奥尔特试剂和比色法测定不同馏分的抗氧化和抗菌性能、精油成分、总酚和黄酮含量。采用薄膜水化法制备纳米颗粒。
气相色谱 - 质谱联用分析表明，六法呢基丙酮是主要的精油成分。[植物名称1]的正丁醇和乙酸乙酯提取物具有最高的抗氧化活性。两种植物的提取物均表现出抗菌活性。确定了提取物对[细菌名称]的最大抑菌圈。粒度分析仪检测到制剂尺寸为140 nm。脂质体的最佳配方包含75 mg卵磷脂、25 mg胆固醇和50 mg草药提取物的比例。尽管纳米颗粒的粒径合适，但脂质体提取物的抗菌效果低于游离形式。
我们的研究结果表明，提取物具有显著的抗菌和抗氧化活性，这归因于其生物活性成分。
