Tufa Teisa, Damianakos Harilaos, Graikou Konstantia, Chinou Loanna
Nat Prod Commun. 2017 Feb;12(2):179-180.
The cyclohexane (Ch) extracts of the roots of five Greek endemic Boraginaceae plants, Onosma kaheirei Teppner, 0. graeca Boiss., 0. erecta Sibth. & Sm., Alkanna sfikasiana Kit Tan, Vold and Strid and Cynoglossum columnae Ten, were investigated for the presence of alkannin/shikonin-related compounds. All species,s except C. columnae and 0. erecta, were found to contain this type of compounds. Seven compounds were obtained after several chromatographic separations from the Ch extracts of the investigated plants: deoxyalkannin (1), 2"-(S)-α-methylbutyrylalkannin (2), isobutyrylalkannin (3), propionylalkannin (4), acetylalkannin (5), β-hydroxyisovalerylalkannin (6), and β,β-dimethylacrylalkannin (7). All structures were identified by ID 1H-/¹³C- and 2D NMR spectroscopy, assisted also by ESI-MS. The extracts and the isolated compounds exhibiting an interesting antimicrobial profile when evaluated for their antimicrobial activity against six Gram-positive and -negative bacteria and three human pathogenic fungi.
对五种希腊特有的紫草科植物，即卡赫雷奥氏滇紫草（Onosma kaheirei Teppner）、希腊滇紫草（O. graeca Boiss.）、直立滇紫草（O. erecta Sibth. & Sm.）、斯菲卡西亚假紫草（Alkanna sfikasiana Kit Tan、Vold和Strid）以及柱序琉璃草（Cynoglossum columnae Ten）根部的环己烷提取物进行了研究，以检测是否存在与紫朱草素/紫草素相关的化合物。结果发现，除柱序琉璃草和直立滇紫草外，所有物种均含有此类化合物。通过对所研究植物的环己烷提取物进行多次色谱分离，得到了七种化合物：脱氧紫朱草素（1）、2''-(S)-α-甲基丁酰紫朱草素（2）、异丁酰紫朱草素（3）、丙酰紫朱草素（4）、乙酰紫朱草素（5）、β-羟基异戊酰紫朱草素（6）和β,β-二甲基丙烯酰紫朱草素（7）。所有结构均通过一维¹H-/¹³C-和二维核磁共振光谱鉴定，并辅以电喷雾电离质谱。当对提取物和分离出的化合物针对六种革兰氏阳性和阴性细菌以及三种人类致病真菌的抗菌活性进行评估时，它们表现出了有趣的抗菌特性。
