Nysanth N S, Sivapriya S L, Natarajan Chitra, Anith K N
Department of Agricultural Microbiology, College of Agriculture, Kerala Agricultural University, Thiruvananthapuram, Kerala 695 522 India.
3 Biotech. 2022 Jun;12(6):140. doi: 10.1007/s13205-022-03205-3. Epub 2022 May 30.
Two novel and rapid in vitro agar plate methods for simultaneous screening of two bacterial isolates for antagonism against multiple fungal phytopathogens in a single agar plate are described. In the modified plus streak method (MPS), in a 10 cm diameter PDA agar plate, two different bacterial isolates were screened against four fungal pathogens. The second method used 20 cm diameter PDA agar plate surface and was termed as the parallel multiple streak (PMS) method. Here, two bacterial isolates were screened for antagonism against ten fungal phytopathogens on a single agar plate surface. Our new methods are rapid and more economical, and could reduce the number of agar plates and the medium required for in vitro screening by several fold, compared to that used in the conventional dual culture plate assay.
本文描述了两种新颖且快速的体外琼脂平板方法，用于在单个琼脂平板上同时筛选两种细菌分离株对多种真菌植物病原体的拮抗作用。在改良的加号划线法（MPS）中，在直径10厘米的马铃薯葡萄糖琼脂（PDA）平板上，针对四种真菌病原体筛选了两种不同的细菌分离株。第二种方法使用直径20厘米的PDA琼脂平板表面，称为平行多重划线（PMS）法。在此方法中，在单个琼脂平板表面筛选两种细菌分离株对十种真菌植物病原体的拮抗作用。我们的新方法快速且更经济，与传统的双平板培养测定法相比，可将体外筛选所需的琼脂平板数量和培养基减少数倍。
