Anith K N, Radhakrishnan N V, Manomohandas T P
Department of Plant Pathology, College of Agriculture, Kerala Agricultural University, Vellayani, Trivandrum, Kerala 695 522, India.
Microbiol Res. 2003;158(2):91-7. doi: 10.1078/0944-5013-00179.
Bacterial antagonists of Phytophthora capsici were isolated from underground shoot portions of rooted cuttings of black pepper. Initially isolates were screened by dual culture on potato dextrose agar and carrot agar. Further, a screening was done on black pepper shoots for supression of lesion caused by the pathogen. Most of the antagonists showed varying levels of antagonism in the dual culture and the shoot assay. Isolate PN-026, showing the highest suppression of lesion development in the shoot assay was found to be the most efficient antagonist in reducing Phytophthora capsici induced nursery wilt of black pepper. This screening involving the host, pathogen, and the antagonist, performed on black pepper shoot (the planting material for this vegetatively propagated crop), could be used as a rapid and reliable method for the isolation of efficient bacterial antagonists of P. capsici.
从黑胡椒扦插生根苗的地下茎部分分离出辣椒疫霉的细菌拮抗剂。最初，通过在马铃薯葡萄糖琼脂和胡萝卜琼脂上进行双重培养来筛选分离物。此外，还对黑胡椒苗进行了病原菌引起的病斑抑制筛选。大多数拮抗剂在双重培养和苗试验中表现出不同程度的拮抗作用。在苗试验中对病斑发展抑制作用最强的分离物PN - 026被发现是减少辣椒疫霉引起的黑胡椒苗圃枯萎病最有效的拮抗剂。这种在黑胡椒苗（这种无性繁殖作物的种植材料）上进行的涉及宿主、病原菌和拮抗剂的筛选，可作为分离高效辣椒疫霉细菌拮抗剂的快速可靠方法。
