Tee R D, Cranston P S, Kay A B
Allergy. 1987 Jan;42(1):12-9. doi: 10.1111/j.1398-9995.1987.tb02181.x.
Chironomid midges are small (2-15 mm) non-biting flies, characteristically seen swarming by water at dusk. Allergens of the "green nimitti" midge, Cladotanytarsus lewisi (Freeman) (Diptera: Chironomidae), a cause of widespread hypersensitivity in the Sudan, were isolated and partially characterized by Sephacryl S200 chromatography. The allergenicity of the fractions was identified by "rocket" autoradiography, RAST inhibition, skin "prick" tests, and the immunoblot technique. The fractions were further analysed by isoelectric focusing and SDS-PAGE. Two major allergens with pI's ranging from 4.3 to 6.0 were identified and had molecular weights of approximately 17,000 and 32,000 daltons, sizes compatible with their being monomeric and dimeric haemoglobins. Since chironomids occur in nuisance numbers worldwide and their haemoglobins have been shown to produce severe hypersensitivity reactions in man, they should be seen as an important potential cause of environmental and occupational allergy.
摇蚊是一种小型（2 - 15毫米）的不叮人的苍蝇，其特征是在黄昏时分在水边成群飞舞。“绿色尼米蒂”摇蚊（Cladotanytarsus lewisi (Freeman)，双翅目：摇蚊科）是苏丹广泛过敏反应的一个病因，其过敏原通过Sephacryl S200色谱法进行了分离和部分特性鉴定。通过“火箭”放射自显影、RAST抑制、皮肤“点刺”试验和免疫印迹技术鉴定了各组分的致敏性。通过等电聚焦和SDS - PAGE对各组分进行了进一步分析。鉴定出两种主要过敏原，其等电点范围为4.3至6.0，分子量约为17,000和32,000道尔顿，其大小与其作为单体和二聚体血红蛋白相符。由于摇蚊在全球大量滋生，并且已证明它们的血红蛋白会在人类中引起严重的过敏反应，因此应将它们视为环境和职业过敏的一个重要潜在原因。
