Tee R D, Cromwell O, Longbottom J L, Cranston P S, Kay A B
Clin Allergy. 1984 Mar;14(2):117-27. doi: 10.1111/j.1365-2222.1984.tb02642.x.
Allergens in extracts of the 'green nimitti' midge, Cladotanytarsus lewisi Freeman (Diptera: Chironomidae), a cause of widespread hypersensitivity in the Sudan, were isolated and partially characterized by assays which depend on the binding of 125I-anti-IgE to allergen-IgE complexes. These methods included RAST inhibition, crossed radioimmunoelectrophoresis (CRIE) and rocket radioimmunoelectrophoresis (RRIE). Following Sephadex G100 chromatography the 'major peak' of allergenicity as determined by RAST inhibition, RRIE and SDS-PAGE was associated with molecules of approximately 17 000 daltons. The peak eluting at Vo contained material of molecular weight 66 000 daltons which also bound 125I-anti-IgE, but had only 61% of the activity of the 'major peak' by RAST inhibition. By isoelectric focusing and RRIE of fractions obtained by chromatofocusing with polybuffer exchanger 94, the 'major peak' was associated with multiple bands with a pI range of 3.5-5.5. These results indicate that the major allergens from C. lewisi are a group of closely related acidic peptides.
“绿色预兆”蠓（Cladotanytarsus lewisi Freeman，双翅目：摇蚊科）的提取物中的过敏原是苏丹广泛过敏反应的一个病因，这些过敏原通过依赖于125I-抗IgE与过敏原-IgE复合物结合的检测方法进行分离和部分表征。这些方法包括放射性变应原吸附试验抑制、交叉放射免疫电泳（CRIE）和火箭放射免疫电泳（RRIE）。经葡聚糖G100层析后，通过放射性变应原吸附试验抑制、火箭放射免疫电泳和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳确定的致敏性“主峰”与分子量约为17000道尔顿的分子相关。在空体积处洗脱的峰含有分子量为66000道尔顿的物质，该物质也能结合125I-抗IgE，但通过放射性变应原吸附试验抑制，其活性仅为“主峰”的61%。通过使用多缓冲交换剂94进行层析聚焦得到的级分进行等电聚焦和火箭放射免疫电泳，“主峰”与多个条带相关，其等电点范围为3.5 - 5.5。这些结果表明，来自刘易斯摇蚊的主要过敏原是一组密切相关的酸性肽。
