使用 ForenSeq™ DNA 签名制备试剂盒对来自墨西哥新莱昂州蒙特雷市（东北部，墨西哥）的墨西哥裔混血人群中的 58 个 STR 标记和 94 个 SNP 进行特征分析。
Characterization of 58 STRs and 94 SNPs with the ForenSeq™ DNA signature prep kit in Mexican-Mestizos from the Monterrey city (Northeast, Mexico).
机构信息
Instituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad #1115, CP 47810, Ocotlán, Jalisco, Mexico.
Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
出版信息
Mol Biol Rep. 2022 Aug;49(8):7601-7609. doi: 10.1007/s11033-022-07575-y. Epub 2022 Jun 3.
BACKGROUND
STR allele frequency databases from populations are necessary to take full advantage of the increased power of discrimination offered by massively parallel sequencing (MPS) platforms.
MATERIAL AND METHODS
For this reason, we sequenced 58 STRs (aSTRs, X-STRs, and Y-STRs) and 94 identity informative SNPs (iiSNPs) on 105 Mestizo (admixed) individuals from Monterrey City (Northeast, Mexico), with the Primer Set-A of the ForenSeq™ DNA Signature Prep Kit.
RESULTS
Most of the STR markers were in Hardy Weinberg equilibrium, with a few exceptions. We found 346 different length-based alleles for these 58 STRs; nevertheless, they became 528 alleles when the sequence was assessed. The combined power of discrimination from autosomal STRs (aSTRs) was -virtually- 100% in both length and sequence-based alleles, while the power of exclusion was 99.9999999976065 and 99.9999999999494%, respectively. Haplotypes based on X-STRs and Y-STRs showed 100% of discriminatory capacity.
CONCLUSIONS
These results provide -for the first time- forensic genomic population data from Mexico necessary for interpretation in kinship and criminal analyses.
背景
为了充分利用大规模平行测序（MPS）平台提供的更高的鉴别能力，需要来自人群的 STR 等位基因频率数据库。
材料和方法
为此，我们对来自墨西哥东北部蒙特雷市的 105 名梅斯蒂索（混血）个体进行了 58 个 STR（aSTR、X-STR 和 Y-STR）和 94 个身份信息 SNP（iiSNP）的测序，使用的是 ForenSeq™ DNA Signature Prep Kit 的 Primer Set-A。
结果
大多数 STR 标记处于 Hardy Weinberg 平衡状态，但也有一些例外。对于这些 58 个 STR，我们发现了 346 个不同的基于长度的等位基因；然而，当评估序列时，它们变成了 528 个等位基因。基于常染色体 STR（aSTR）的鉴别能力在长度和序列等位基因上几乎达到 100%，而排除率分别为 99.9999999976065%和 99.9999999999494%。基于 X-STR 和 Y-STR 的单倍型显示出 100%的鉴别能力。
结论
这些结果首次提供了来自墨西哥的法医基因组人群数据，这些数据对于亲属关系和犯罪分析中的解释是必要的。
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