Department of Chemistry, The University of Azad Jammu and Kashmir, Muzaffarabad, Pakistan.
Department of Chemistry, University of Kotli, Kotli, Azad Jammu & Kashmir, Pakistan.
PLoS One. 2022 Jun 3;17(6):e0269444. doi: 10.1371/journal.pone.0269444. eCollection 2022.
The antioxidant capacity of extracts of different parts of Cucurbitaceae vegetables was evaluated by DPPH (2, 2-diphenyl-1-picrylhydrazyl) and ABTS (2, 2'-azino bis (ethyl benzothiazoline 6)-sulphonic acid) methods. Total phenolic content (TPC) and total flavonoid content (TFC) were also determined. The correlation of TPC, TFC, DPPH, and ABTS in different extracts of Cucurbitaceae vegetables was analyzed. The peel extracts of studied vegetables had the highest TPC, (C. grandis 3.00±0.86, T. cucumerina 3.24±0.70 and C. moschata 3.12±0.06 mg gallic acid equivalent (GAE) g-1 DW) and TFC (C. grandis 18.96±1.5, T. cucumerina 13.92±1.41 and C. moschata 15.31±0.97 mg rutin equivalent (RE) g-1 DW). The maximum antioxidant potential was obtained by the ABTS method in peel extracts of C. grandis (78.7%) and C. moschata (63.5%) while in pulp extract of T. cucumerina (50.1%) at 10 μg/mL. The percent radical scavenging activity (% RSA) by the DPPH method found maximum for peel and pulp of C. grandis (45.15 and 45.15%, respectively) and peel of T. cucumerina (45.15%) and C. moschata (34.15%). The EC50 obtained in the ABTS method was 0.54 and 7.15 μg/mL for C. grandis and C. moschata, respectively while 0.81 μg/mL for the pulp of T. cucumerina compared to standard ascorbic acid (1.05 μg/mL). The EC50 calculated in the DPPH method was 11.78 μg/mL, 13.34 μg/mL, and 21.00 μg/mL for C. grandis, T. cucumerina, and C. moschata peel respectively compared to the standard Butylated hydroxytoluene (BHT). Among each variable, the correlation between ABTS and TPC provided the highest positive correlation (r = 0.998, p< 0.05) in peel extracts.
采用 DPPH（2,2-二苯基-1-苦基肼）和 ABTS（2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）二铵盐）法评价了葫芦科蔬菜不同部位提取物的抗氧化能力。还测定了总酚含量（TPC）和总黄酮含量（TFC）。分析了葫芦科蔬菜不同提取物中 TPC、TFC、DPPH 和 ABTS 的相关性。研究蔬菜的果皮提取物具有最高的 TPC（C. grandis 3.00±0.86、T. cucumerina 3.24±0.70 和 C. moschata 3.12±0.06mg 没食子酸当量（GAE）g-1 DW）和 TFC（C. grandis 18.96±1.5、T. cucumerina 13.92±1.41 和 C. moschata 15.31±0.97mg 芦丁当量（RE）g-1 DW）。C. grandis 和 C. moschata 的果皮提取物中 ABTS 法得到的最大抗氧化能力分别为 78.7%和 63.5%，而 T. cucumerina 的果肉提取物在 10μg/mL 时为 50.1%。DPPH 法的自由基清除活性百分比（% RSA），C. grandis 的果皮和果肉（分别为 45.15%和 45.15%）和 T. cucumerina 的果皮（45.15%）和 C. moschata（34.15%）均为最高。ABTS 法测定的 EC50 分别为 0.54μg/mL 和 7.15μg/mL，C. grandis 和 C. moschata 果肉为 0.81μg/mL，而 T. cucumerina 果肉为 1.05μg/mL。DPPH 法测定的 EC50 分别为 11.78μg/mL、13.34μg/mL 和 21.00μg/mL，C. grandis、T. cucumerina 和 C. moschata 果皮的标准为 Butylated hydroxytoluene（BHT）。在每个变量中，ABTS 和 TPC 之间的相关性在果皮提取物中提供了最高的正相关（r=0.998，p<0.05）。
