Green R A, LaFollette K A, Greig B
Am J Vet Res. 1987 Mar;48(3):496-8.
Hexadimethrine bromide was evaluated as a heparin-neutralizing agent in a simple modification of the activated partial thromboplastin time test in canine plasma. Addition of various amounts of heparin in vitro to canine plasma indicated that heparin could be neutralized by adding 0.5 micrograms of hexadimethrine bromide 15 s before CaCl2 was added to the reaction mixture of the activated partial thromboplastin time test. In 8 dogs given (subcutaneous injection) 500 USP units of sodium heparin/kg, marked individual variations in clotting time prolongations were observed over the 12-hour period of study. The hexadimethrine bromide modification effectively neutralized the heparin-related clotting time prolongations to values that were not significantly different from base-line (preheparin) activated partial thromboplastin time values. The modification seems to be useful in confirming the presence of heparin and in monitoring heparin therapy in dogs.
在对犬血浆活化部分凝血活酶时间试验进行简单改良的过程中，对溴化己二甲铵作为肝素中和剂进行了评估。在体外向犬血浆中添加不同量的肝素表明，在向活化部分凝血活酶时间试验的反应混合物中添加氯化钙前15秒加入0.5微克溴化己二甲铵，可中和肝素。在8只皮下注射500 USP单位/千克肝素钠的犬中，在12小时的研究期间观察到凝血时间延长存在明显的个体差异。溴化己二甲铵改良法有效地将与肝素相关的凝血时间延长中和至与基线（肝素前）活化部分凝血活酶时间值无显著差异的值。该改良法似乎有助于确认犬体内肝素的存在并监测肝素治疗情况。
