Center for Neonatal Screening, Department of Congenital Disorders-Clinical Mass Spectrometry Statens Serum Institut, Artillerivej 5, DK-2300, Copenhagen S, Denmark.
National Centre for Register-Based Research, Department of Economics and Business Economics, Aarhus University, Fuglesangs Allé 26, Building 2640 Aarhus V, DK-8210, Aarhus, Denmark.
BMC Res Notes. 2022 Jun 3;15(1):194. doi: 10.1186/s13104-022-06077-1.
We developed an assay to measure the concentration of 25 hydroxyvitamin D and D in protein extracts derived from stored neonatal dried blood spots. During this study, we postulated that these samples had been contaminated with exogenous vitamin D metabolites because of the addition of bovine serum albumin (BSA) as part of an extraction step undertaken 7 years earlier. The aim of the current study was to develop methods in order to adjust for this contamination.
We identified between-plate variations in 25 hydroxyvitamin D and D concentrations which suggested the presence of three different BSA batches. Based on repeat extraction (without the addition of BSA) and testing of 395 samples, we developed models to correct for the exogenous 25 hydroxyvitamin D and D The regression models were Diff = - 8.2 + 1.8* Diff for low contamination, Diff = 23.8 + 1.7* Diff for middle contamination, and Diff = 14.3 + 3.0* Diff for high contamination. After these corrections, the three subsamples had comparable distributions within the expected range for both 25 hydroxyvitamin D and D.
我们开发了一种测定方法，用于测量存储的新生儿干血斑蛋白提取物中 25 羟基维生素 D 和 D 的浓度。在这项研究中，我们假设这些样本因添加牛血清白蛋白（BSA）而受到外源性维生素 D 代谢物的污染，因为在 7 年前的一个提取步骤中添加了 BSA。本研究的目的是开发方法以调整这种污染。
我们发现 25 羟基维生素 D 和 D 浓度的板间差异表明存在三种不同的 BSA 批次。基于重复提取（不添加 BSA）和 395 个样本的测试，我们开发了校正外源性 25 羟基维生素 D 和 D 的模型。回归模型为低污染时 Diff = -8.2 + 1.8 * Diff，中污染时 Diff = 23.8 + 1.7 * Diff，高污染时 Diff = 14.3 + 3.0 * Diff。经过这些校正后，三个亚样本在 25 羟基维生素 D 和 D 的预期范围内具有可比的分布。
