Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, China; Fruit Crop Genetic Improvement and Seedling Propagation Engineering Center of Jiangsu Province, Nanjing, 210095, China.
Institute of Genomics for Crop Abiotic Stress Tolerance, Texas Tech University, Lubbock, TX, 79409, USA; Institute of Genomics for Crop Abiotic Stress Tolerance, Texas Tech University, Lubbock, TX, 79409, USA.
Environ Pollut. 2022 Aug 15;307:119561. doi: 10.1016/j.envpol.2022.119561. Epub 2022 Jun 1.
Improper application of copper-based fungicides has made copper stress critical in viticulture, necessitating the need to identify substances that can mitigate it. In this study, leaves of 'Shine Muscat' ('SM') grapevine seedlings were treated with CuSO solution (10 mM/L), CuSO + 5-aminolevulinic acid (ALA) (50 mg/L), and distilled water to explore the mitigation effect of ALA. Physiological assays demonstrated that ALA effectively reduced malondialdehyde accumulation and increased peroxidase and superoxide dismutase activities in grapevine leaves under copper stress. Copper ion absorption, transport pathways, chlorophyll metabolism pathways, photosynthetic system, and antioxidant pathways play key roles in ALA alleviated-copper stress. Moreover, expression changes in genes, such as CHLH, ALAD, RCA, and DHAR, play vital roles in these processes. Furthermore, abscisic acid reduction caused by NCED down-regulation and decreased naringenin, leucopelargonidin, and betaine contents confirmed the alleviating effect of ALA. Taken together, these results reveal how grapevine responds to copper stress and the alleviating effects of ALA, thus providing a novel means of alleviating copper stress in viticulture.
铜基杀菌剂的不当使用使得铜胁迫成为葡萄栽培中的一个关键问题，因此需要寻找能够缓解铜胁迫的物质。在本研究中，用 CuSO4 溶液（10 mM/L）、CuSO4+5-氨基乙酰丙酸（ALA）（50 mg/L）和蒸馏水处理“Shine Muscat”（SM）葡萄幼苗叶片，以探索 ALA 的缓解作用。生理测定表明，ALA 能有效减少铜胁迫下葡萄叶片中丙二醛的积累，提高过氧化物酶和超氧化物歧化酶的活性。铜离子吸收、运输途径、叶绿素代谢途径、光合作用系统和抗氧化途径在 ALA 缓解铜胁迫中起关键作用。此外，CHLH、ALAD、RCA 和 DHAR 等基因的表达变化在这些过程中也起着重要作用。此外，下调 NCED 导致脱落酸减少，以及柚皮素、莱菔素和甜菜碱含量降低，证实了 ALA 的缓解作用。综上所述，这些结果揭示了葡萄对铜胁迫的反应以及 ALA 的缓解作用，为缓解葡萄栽培中的铜胁迫提供了一种新方法。
