Association of Interleukin-1 Beta and Interleukin-1 Receptor Antagonist Gene Polymorphisms and Plasma Levels with Diabetic Nephropathy.

OBJECTIVE

We investigated the relationships between interleukin- (IL-) 1 and IL-1 receptor antagonist (IL-1Ra) gene polymorphism and plasma levels in patients with diabetic nephropathy (DN).

METHODS

The genotype and allele frequency distribution of IL-1 and IL-1Ra in 61 patients with DN and 48 healthy controls (HCs) were determined by kompetitive allele-specific PCR (KASP), and the plasma concentrations of IL-1 and IL-1Ra in DN patients and HCs were measured by enzyme-linked immunosorbent assays (ELISA).

RESULTS

Significant differences were detected in the distribution of IL-1 (-511C/T) genotype and allele frequencies between the DN and HC groups ( < 0.05), with the T genotype being more frequent in DN patients than HCs (OR = 2.84, 95% CI: 1.489-5.416). The IL-1 (+3953C/T) and IL-1Ra (+8006C/T) genotypes and allele frequencies were not significantly different between the two groups ( > 0.05). The plasma IL-1 level was significantly higher ( < 0.01), while the plasma IL-1Ra concentration was significantly lower in the DN group than the HC group ( < 0.05). Furthermore, the plasma IL-1 level was significantly different between IL-1 (-511C/T) locus variants ( < 0.05).

CONCLUSION

The IL-1 (-511C/T) gene polymorphism was significantly associated with DN risk in the population of northern Guangxi, China, and the T allele maybe responsible for genetic susceptibility to DN.