Maruya Keith A, Lao Wenjian, Vandervort Darcy R, Fadness Richard, Lyons Michael, Mehinto Alvine C
Southern California Coastal Water Research Project Authority, Costa Mesa, CA, 92626, USA.
California Regional Water Quality Control Board, North Coast Region, Santa Rosa, CA, 95403, USA.
Heliyon. 2022 May 25;8(5):e09534. doi: 10.1016/j.heliyon.2022.e09534. eCollection 2022 May.
To broaden the scope of contaminants monitored in human-impacted riverine systems, water, sediment, and treated wastewater effluent were analyzed using receptor-based cell assays that provide an integrated response to chemicals based on their mode of biological activity. Samples were collected from three California (USA) watersheds with varying degrees of urbanization and discharge from municipal wastewater treatment plants (WWTPs). To complement cell assay results, samples were also analyzed for a suite of contaminants of emerging concern (CECs) using gas and liquid chromatography-mass spectrometry (GC- and LC-MS/MS). For most water and sediment samples, bioassay equivalent concentrations for estrogen and glucocorticoid receptor assays (ER- and GR-BEQs, respectively) were near or below reporting limits. Measured CEC concentrations compared to monitoring trigger values established by a science advisory panel indicated minimal to moderate concern in water but suggested that select pesticides (pyrethroids and fipronil) had accumulated to levels of greater concern in river sediments. Integrating robust, standardized bioanalytical tools such as the ER and GR assays utilized in this study into existing chemical-specific monitoring and assessment efforts will enhance future CEC monitoring efforts in impacted riverine systems and coastal watersheds.
为了扩大在受人类影响的河流系统中监测的污染物范围，我们使用基于受体的细胞分析方法对水、沉积物和处理后的废水排放物进行了分析，该方法根据化学物质的生物活性模式对其提供综合响应。样本采集自美国加利福尼亚州的三个流域，这些流域的城市化程度和城市污水处理厂（WWTPs）的排放量各不相同。为了补充细胞分析结果，还使用气相色谱-质谱联用仪和液相色谱-串联质谱仪（GC-和LC-MS/MS）对一系列新兴关注污染物（CECs）进行了分析。对于大多数水和沉积物样本，雌激素和糖皮质激素受体分析的生物测定等效浓度（分别为ER-和GR-BEQs）接近或低于报告限值。将测得的CEC浓度与科学咨询小组确定的监测触发值进行比较，结果表明水中的关注程度最低到中等，但表明某些农药（拟除虫菊酯和氟虫腈）在河流沉积物中的积累达到了更高的关注水平。将本研究中使用的强大、标准化的生物分析工具（如ER和GR分析）整合到现有的针对特定化学物质的监测和评估工作中，将加强未来对受影响的河流系统和沿海流域中CECs的监测工作。
