Zhang Shiqiang, Tao Wanyu, Han Jing-Dong J
Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Center for Quantitative Biology (CQB), Peking University, Beijing 100871, China.
CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China.
Comput Struct Biotechnol J. 2022 May 18;20:2434-2441. doi: 10.1016/j.csbj.2022.05.032. eCollection 2022.
Gametogenesis, including spermatogenesis and oogenesis, are unique differentiation processes involving extraordinarily complex and precise regulatory mechanisms that require the interactions of multiple cell types, hormones, paracrine factors, genes and epigenetic regulators, and extensive chromatin 3D structure re-organization. In recent years, the development of 3D genome technology represented by Hi-C, enabled mapping of the 3D re-organization of chromosomes during zygogenesis at an unprecedented resolution. The 3D remodeling is achieved by folding chromatin into loops, topologically associating domains (TADs), and compartments (A and B), which ultimately affect transcriptional activity. In this review, we summarize the research progresses and findings on chromatin 3D structure changes during spermatogenesis and oogenesis.
配子发生，包括精子发生和卵子发生，是独特的分化过程，涉及极其复杂和精确的调控机制，这需要多种细胞类型、激素、旁分泌因子、基因和表观遗传调节因子的相互作用，以及广泛的染色质三维结构重组。近年来，以Hi-C为代表的三维基因组技术的发展，使得能够以前所未有的分辨率绘制合子发生过程中染色体的三维重组图谱。三维重塑是通过将染色质折叠成环、拓扑相关结构域（TADs）和区室（A和B）来实现的，这最终会影响转录活性。在本综述中，我们总结了精子发生和卵子发生过程中染色质三维结构变化的研究进展和发现。
