Younas Anam, Hussain Liaqat, Shabbir Arham, Asif Muhammad, Hussain Musaddique, Manzoor Faiza
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, Pakistan.
Institute of Pharmacy, Faculty of Pharmaceutical and Allied Health Sciences, Lahore College for Women University, Lahore, Pakistan.
Evid Based Complement Alternat Med. 2022 May 26;2022:1397060. doi: 10.1155/2022/1397060. eCollection 2022.
Polycystic ovarian syndrome is a multidisciplinary endocrinopathy of reproductive-aged women that provokes insulin resistance, hyperandrogenism, cardiovascular problems, obesity, and menstrual complications. The present study was designed to investigate the effectiveness of ethanolic extract of in letrozole-induced PCOS young adult female rats. HPLC was carried out to find the phenolic and flavonoid content of the ethanolic extract of . Twenty-five female rats were taken and initially divided into two groups: group I (control group) and group II (PCOS group). PCOS was induced by letrozole given orally by gavage. Body weight was recorded weekly and vaginal cytology was analyzed daily. After induction of disease, the PCOS group is further divided into four groups ( = 5): group II (positive control with PCOS), group III (metformin 20 mg/kg treated group), group IV (ethanolic extract of 500 mg/kg treated group), and group V (metformin plus extract). At the end of experimental period, the blood sample of each rat was collected and serum was separated by centrifugation. Afterwards hormonal analysis, lipid profile and liver functioning tests were performed. Ovaries were removed and preserved for histopathological findings while the liver of each rat was stored for the determination of antioxidant potential assessment. was found to possess quercetin as one of the major flavonoid phytoconstituents. The plant extract exhibited its beneficial effects by restoring hormonal balance, lipid profile, and liver functioning markers. Treatment with . reduced body weight, resolved ovarian cysts, and showed positive effects on follicular growth. Treatment with plant also increased the levels of antioxidant enzymes. This study validates the potential of for the amelioration of metabolic, as well as, hormonal disturbances that occurred in PCOS.
多囊卵巢综合征是一种发生于育龄女性的多学科内分泌疾病，可引发胰岛素抵抗、高雄激素血症、心血管问题、肥胖和月经紊乱。本研究旨在调查[植物名称]乙醇提取物对来曲唑诱导的多囊卵巢综合征成年雌性大鼠的有效性。采用高效液相色谱法测定[植物名称]乙醇提取物中的酚类和黄酮类成分。选取25只雌性大鼠，最初分为两组：第一组（对照组）和第二组（多囊卵巢综合征组）。通过灌胃口服来曲唑诱导多囊卵巢综合征。每周记录体重，每天分析阴道细胞学。疾病诱导后，多囊卵巢综合征组进一步分为四组（每组n = 5）：第二组（多囊卵巢综合征阳性对照组）、第三组（二甲双胍20 mg/kg治疗组）、第四组（[植物名称]乙醇提取物500 mg/kg治疗组）和第五组（二甲双胍加[植物名称]提取物组）。实验期结束时，采集每只大鼠的血样，通过离心分离血清。随后进行激素分析、血脂谱和肝功能测试。取出卵巢并保存以进行组织病理学检查，同时保存每只大鼠的肝脏以测定抗氧化潜力评估。发现[植物名称]含有槲皮素作为主要黄酮类植物成分之一。该植物提取物通过恢复激素平衡、血脂谱和肝功能指标发挥其有益作用。用[植物名称]治疗可减轻体重、消除卵巢囊肿，并对卵泡生长产生积极影响。用该植物治疗还可提高抗氧化酶水平。本研究证实了[植物名称]改善多囊卵巢综合征中发生的代谢和激素紊乱的潜力。
