Department of Pediatrics, Renmin Hospital of Wuhan University, Wuhan, China.
Department of Psychiatry, Renmin Hospital of Wuhan University, Wuhan, China.
Epilepsy Behav. 2022 Jul;132:108738. doi: 10.1016/j.yebeh.2022.108738. Epub 2022 Jun 3.
Depression is one of the most common comorbid psychiatric condition associated with epilepsy. It has a negative impact on the patient's quality of life. However, the underlying molecular mechanisms leading to depression are currently unclear. The aim of this study was to determine the hub genes associated with epilepsy and depression.
Gene expression profiles (GSE47752 and GSE20388) were downloaded from the gene expression omnibus (GEO) database. Differentially expressed genes (DEGs) for epilepsy and depression groups were separately searched. Subsequently, network analyses methods were employed to establish protein-protein interaction (PPI) networks, and to perform Gene Ontology (GO) terms and pathway enrichment analyses for co-expressed DEGs.
A total of 772 genes were upregulated in patients with epilepsy whereas 91 genes were up-regulated in patients with depression. In addition, 1304 genes were down-regulated in epilepsy whereas 141 genes were down-regulated in patients with depression. Among co-expressed DEGs, 5 DEGs were up-regulated and 19 were down-regulated. Further analysis revealed that the co-expressed DEGs were involved in regulation of vasculature development, regulation of angiogenesis, glutamate receptor signaling pathway, cellular response to interleukin-1 and positive regulation of protein kinase B signaling. The Arc and Homer1 genes were identified as the common candidate genes involved in the pathogenesis of epilepsy and depression.
Arc and Homer1 may contribute to the comorbidity of epilepsy and depression.
抑郁症是与癫痫相关的最常见合并精神疾病之一。它对患者的生活质量有负面影响。然而，导致抑郁症的潜在分子机制目前尚不清楚。本研究旨在确定与癫痫和抑郁症相关的关键基因。
从基因表达综合数据库（GEO）下载基因表达谱（GSE47752 和 GSE20388）。分别搜索癫痫和抑郁症组的差异表达基因（DEGs）。随后，采用网络分析方法建立蛋白质-蛋白质相互作用（PPI）网络，并对共表达的 DEGs 进行基因本体论（GO）术语和通路富集分析。
与癫痫患者相比，共有 772 个基因上调，而 91 个基因在抑郁症患者中上调。此外，在癫痫患者中，有 1304 个基因下调，而在抑郁症患者中，有 141 个基因下调。在共表达的 DEGs 中，有 5 个基因上调，19 个基因下调。进一步分析表明，共表达的 DEGs 参与了血管发育的调节、血管生成的调节、谷氨酸受体信号通路、细胞对白细胞介素-1 的反应和蛋白激酶 B 信号的正调控。Arc 和 Homer1 基因被确定为参与癫痫和抑郁症发病机制的共同候选基因。
Arc 和 Homer1 可能导致癫痫和抑郁症的共病。
