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肠球菌属中保守的假定细胞壁结合域的 H、C 和 N 共振分配。

H, C, and N resonance assignments of a conserved putative cell wall binding domain from Enterococcus faecalis.

机构信息

School of Biosciences, University of Sheffield, Firth Court, Western Bank, S10 2TN, Sheffield, UK.

出版信息

Biomol NMR Assign. 2022 Oct;16(2):247-251. doi: 10.1007/s12104-022-10087-2. Epub 2022 Jun 4.

DOI:10.1007/s12104-022-10087-2
PMID:35665899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9510096/
Abstract

Enterococcus faecalis is a major causative agent of hospital acquired infections. The ability of E. faecalis to evade the host immune system is essential during pathogenesis, which has been shown to be dependent on the complete separation of daughter cells by peptidoglycan hydrolases. AtlE is a peptidoglycan hydrolase which is predicted to bind to the cell wall of E. faecalis, via six C-terminal repeat sequences. Here, we report the near complete assignment of one of these six repeats, as well as the predicted backbone structure and dynamics. This data will provide a platform for future NMR studies to explore the ligand recognition motif of AtlE and help to uncover its potential role in E. faecalis virulence.

摘要

粪肠球菌是医院获得性感染的主要病原体。在发病机制中，粪肠球菌逃避宿主免疫系统的能力至关重要，这已被证明依赖于肽聚糖水解酶将子细胞完全分离。AtlE 是一种肽聚糖水解酶，据预测通过 6 个 C 末端重复序列与粪肠球菌的细胞壁结合。在这里，我们报告了其中一个重复序列的几乎完整分配，以及预测的骨架结构和动力学。该数据将为未来的 NMR 研究提供一个平台，以探索 AtlE 的配体识别基序，并有助于揭示其在粪肠球菌毒力中的潜在作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8315/9510096/36f307539765/12104_2022_10087_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8315/9510096/60d000963b57/12104_2022_10087_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8315/9510096/70e85b0797a9/12104_2022_10087_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8315/9510096/36f307539765/12104_2022_10087_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8315/9510096/60d000963b57/12104_2022_10087_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8315/9510096/70e85b0797a9/12104_2022_10087_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8315/9510096/36f307539765/12104_2022_10087_Fig3_HTML.jpg

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本文引用的文献

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Decoration of the enterococcal polysaccharide antigen EPA is essential for virulence, cell surface charge and interaction with effectors of the innate immune system.肠球菌多糖抗原 EPA 的装饰对于其毒力、细胞表面电荷以及与先天免疫系统效应子的相互作用是必不可少的。
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A genomic virulence reference map of Enterococcus faecalis reveals an important contribution of phage03-like elements in nosocomial genetic lineages to pathogenicity in a Caenorhabditis elegans infection model.粪肠球菌的基因组毒力参考图谱揭示了在秀丽隐杆线虫感染模型中，医院遗传谱系中类噬菌体03元件对致病性的重要贡献。
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Protein backbone and sidechain torsion angles predicted from NMR chemical shifts using artificial neural networks.使用人工神经网络从 NMR 化学位移预测蛋白质主链和侧链扭转角。
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The rise of the Enterococcus: beyond vancomycin resistance.肠球菌的兴起：超越万古霉素耐药性。
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