Intelligent Minimally-Invasive Device Center, National Chung Hsing University, Taichung, 40227, Taiwan; Department of Mechanical Engineering, National Chung-Hsing University, Taichung, 40227, Taiwan.
Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, 10002, Taiwan.
Biosens Bioelectron. 2022 Oct 1;213:114440. doi: 10.1016/j.bios.2022.114440. Epub 2022 May 30.
It has been found that the direct/total bilirubin ratio (D/T-BIL) is related to the survival rate of COVID-19 pneumonia. The presence of an excessive amount of bilirubin in human blood also causes liver and neurological damage, leading to death. Therefore, upon considering the adverse impact of the presence of excessive bilirubin in human blood, it has become highly imperative to detect bilirubin in a fast and label-free manner. Herein, we designed and constructed a random-crossed-woodpile nanostructure from silver nanowires to form a 3-dimensional plasmonic hotspot-rich (3D-PHS) nanostructure and successfully used it to detect direct bilirubin (D-BIL) in human blood in a label-free manner. The 3D-PHS nanochip provides rich spatial hot spots that are simultaneously responsive to SERS and SPEF effects and consequently, successfully used to measure and characterize D-BIL with a detection limit of ∼10 nM, requiring only 10μL of human serum for rapid screening, which is the first time D-BIL has been detected in a clinically relevant range. This demonstrates a simple, label-free, pretreatment-free potential biosensing technology that can be used in health care units, and further, in the efficient detection of point-of-care testing with a portable spectrometer.
已经发现直接/总胆红素比值（D/T-BIL）与 COVID-19 肺炎的存活率有关。人体血液中胆红素含量过高也会导致肝和神经损伤，从而导致死亡。因此，考虑到人体血液中胆红素过多的不良影响，快速、无标记地检测胆红素变得至关重要。在此，我们设计并构建了一种由银纳米线组成的随机交叉木堆纳米结构，形成了一个具有丰富等离子体热点的 3 维（3D）结构，并成功地用于无标记检测人血中的直接胆红素（D-BIL）。3D-PHS 纳米芯片提供了丰富的空间热点，同时对 SERS 和 SPEF 效应有响应，因此成功地用于测量和表征 D-BIL，检测限约为 10 nM，仅需 10 μL 人血清即可快速筛选，这是首次在临床相关范围内检测到 D-BIL。这证明了一种简单、无标记、无需预处理的潜在生物传感技术，可用于医疗保健单位，并可进一步用于带有便携式分光光度计的即时检测的高效检测。
