Protrustech Co., Ltd., 3F.-1, No.293, Sec. 3, Dongmen Rd. East District, Tainan City 701, Taiwan.
Department of Plant Pathology, National Chung-Hsing University, Taichung 402, Taiwan.
Molecules. 2021 Jan 8;26(2):281. doi: 10.3390/molecules26020281.
In this manuscript, silver nanowire 3D random crossed-wire woodpile (3D-RCW) nanostructures were designed and prepared. The 3D-RCW provides rich "antenna" and "hot spot" effects that are responsive for surface-enhanced Raman scattering (SERS) effects and plasmon-enhanced fluorescence (PEF). The optimal construction mode for the 3D-RCW, based on the ratio of silver nanowire and control compound R6G, was explored and established for use in PEF and SERS analyses. We found that the RCW nanochip capable of emission and Raman-enhanced detections uses micro levels of analysis volumes. Consequently, and SERS and PEF of pesticides (thiram, carbaryl, paraquat, fipronil) were successfully measured and characterized, and their detection limits were within 5 μM~0.05 µM in 20 µL. We found that the designed 3D plasmon-enhanced platform cannot only collect the SERS of pesticides, but also enhance the fluorescence of a weak emitter (pesticides) by more than 1000-fold via excitation of the surface plasmon resonance, which can be used to extend the range of a fluorescence biosensor. More importantly, solid-state measurement using a 3D-RCW nanoplatform shows promising potential based on its dual applications in creating large SERS and PEF enhancements.
在本文中，设计并制备了银纳米线三维随机交叉线木堆（3D-RCW）纳米结构。3D-RCW 提供了丰富的“天线”和“热点”效应，可用于表面增强拉曼散射（SERS）效应和等离子体增强荧光（PEF）。基于银纳米线和对照化合物 R6G 的比例，探索并建立了 3D-RCW 的最佳构建模式，用于 PEF 和 SERS 分析。我们发现，能够进行发射和拉曼增强检测的 RCW 纳米芯片使用微级分析体积。因此，成功地测量和表征了农药（福美双、西玛津、百草枯、氟虫腈）的 SERS 和 PEF，其检测限在 20 μL 内为 5 μM~0.05 μM。我们发现，所设计的 3D 等离子体增强平台不仅可以收集农药的 SERS，还可以通过表面等离子体共振的激发将弱发射体（农药）的荧光增强 1000 多倍，从而可以扩展荧光生物传感器的范围。更重要的是，基于其在创建大的 SERS 和 PEF 增强方面的双重应用，使用 3D-RCW 纳米平台进行固态测量显示出了有前景的潜力。
