Bougis P E, Teissié J, Rochat H, Piéroni G, Verger R
Biochem Biophys Res Commun. 1987 Mar 13;143(2):506-11. doi: 10.1016/0006-291x(87)91382-9.
Surface fluorescence spectroscopy was used to study mixed phospholipid-cardiotoxin monomolecular films. Using polarized incident light we were able to detect the intrinsic fluorescence emission at a wavelength of 339 nm of the single Trp11 in the cardiotoxin molecule. Its fluorescence intensity increased continuously upon film compression up to a maximal value reached at 42 mN x m-1 in surface pressure. A relative polarization index can be used as an indication of the orientation of the indole ring. A transition at around 25 +/- 5 mN x m-1 in the orientation of the Trp11 relative to the plane of the interface was clearly evidenced during film compression, in agreement with our independent data showing a flipping of the whole toxin molecule (Bougis et al., (1981), Biochemistry 20, 4915-4920).
