Kim I H, Lee H J
Biochem Int. 1987 Jan;14(1):103-10.
Isolated pig heart mitochondria were found to form phosphocreatine continuously at the rate of 12.5 +/- 1.8 nmol per min per mg of the mitochondrial protein in the respiration medium without externally added adenine nucleotides, and its formation rate showed a concentration dependency with respect to creatine and phosphate. The synthesis of phosphocreatine was completely inhibited by antimycin, FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone), and atractyloside. However, oligomycin had no effect on the rate of phosphocreatine formation. These results are discussed in terms of a model that heart mitochondrial creatine kinase is functionally coupled to the oxidative phosphorylating system via the action of the adenine nucleotide translocase.
在没有外源添加腺嘌呤核苷酸的呼吸介质中，发现分离的猪心脏线粒体以每分钟每毫克线粒体蛋白12.5±1.8纳摩尔的速率持续形成磷酸肌酸，其形成速率显示出对肌酸和磷酸盐的浓度依赖性。抗霉素、FCCP（羰基氰-对-三氟甲氧基苯腙）和苍术苷完全抑制了磷酸肌酸的合成。然而，寡霉素对磷酸肌酸的形成速率没有影响。根据心脏线粒体肌酸激酶通过腺嘌呤核苷酸转位酶的作用与氧化磷酸化系统功能偶联的模型对这些结果进行了讨论。
