Vaysse J, Pilardeau P, Gattegno L, Garnier M
Br J Haematol. 1987 Mar;65(3):361-5. doi: 10.1111/j.1365-2141.1987.tb06869.x.
During treatment of human red cells with phospholipase A2 from bee venom, a linear increase of the MCV and of the osmotic fragility occurs in parallel with the cleavage of the accessible phospholipids. However, even after maximal hydrolysis, i.e. degradation of up to 65% of the phosphatidylcholines and up to 6% of the phosphatidyl-ethanolamines, almost no haemolysis is observed and the median corpuscular fragility is only 7% higher than that of control cells incubated without enzyme. Addition of albumin to the medium results in an important rise of the susceptibility to hypotonic saline solutions. Osmotic fragility curves obtained with red cells submitted to mild phospholipase action show evidence of subpopulations of cells with various sensitivities to osmotic lysis. This phenomenon can be partly explained by the heterogeneity of the cleavage intensity among the cell population. This hypothesis is supported by the studies of the lipid composition of phospholipase treated red cells fractionated according to their sensitivity to hypotonic lysis or to their size.
在用蜂毒中的磷脂酶A2处理人红细胞的过程中，平均红细胞体积（MCV）和渗透脆性呈线性增加，这与可及磷脂的裂解同时发生。然而，即使在最大程度水解后，即高达65%的磷脂酰胆碱和高达6%的磷脂酰乙醇胺被降解，几乎也未观察到溶血现象，且平均红细胞脆性仅比未用酶孵育的对照细胞高7%。向培养基中添加白蛋白会导致对低渗盐溶液的敏感性显著增加。用经受轻度磷脂酶作用的红细胞获得的渗透脆性曲线显示出对渗透裂解具有不同敏感性的细胞亚群的证据。这种现象可以部分地通过细胞群体中裂解强度的异质性来解释。根据对低渗裂解的敏感性或细胞大小对经磷脂酶处理的红细胞进行分级分离，并对其脂质组成进行研究，这一假设得到了支持。
