经蜂毒磷脂酶A2处理的兔红细胞的特性
Properties of rabbit erythrocytes treated with phospholipase A2 from bee venom.
作者信息
Vaysse J, Pilardeau P, Garnier M
出版信息
Comp Biochem Physiol A Comp Physiol. 1986;83(4):715-9. doi: 10.1016/0300-9629(86)90715-2.
PMID:2870863
Abstract
In isotonic saline-buffered conditions, phospholipase A2 from bee venom cleaves up to 65% of the phosphatidylcholine of the rabbit RBC membrane without causing significant hemolysis; however, the volume and the osmotic fragility of the treated RBC is modified. The osmotic behaviour of PLA-treated RBC after reincubation in autologous plasma suggests an heterogeneity in the enzymatic attack among the RBC population. In vivo RBC survival is strongly impaired by PLA treatment.
摘要
在等渗盐水缓冲条件下，蜂毒中的磷脂酶A2可裂解兔红细胞膜中高达65%的磷脂酰胆碱，而不会引起明显的溶血；然而，处理后的红细胞的体积和渗透脆性会发生改变。经磷脂酶A处理的红细胞在自体血浆中再孵育后的渗透行为表明，红细胞群体之间的酶促攻击存在异质性。磷脂酶A处理会严重损害红细胞在体内的存活。
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