School of Life Sciences, Huizhou University, Huizhou 510607, China.
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, South China Normal University, Guangzhou 510631, China.
J Agric Food Chem. 2022 Jun 22;70(24):7441-7446. doi: 10.1021/acs.jafc.2c02380. Epub 2022 Jun 7.
is a pathogenic fungus that causes false smut disease in rice during the flowering stage through stamen filaments. Currently, there is a need to develop safe and effective antifungal agents for the control of this disease. In our preliminary experiments, we found that MTE-1, a new trisaccharide ester, exhibits significant inhibitory activity against . Hence, the effects and inhibitory mechanism of MTE-1 in were investigated. Results showed that the MTE-1 inhibited the hyphae growth of with an IC of 5.67 μg/mL. Similarly, MTE-1 disrupted the endomembrane system in , especially the plasma membrane, mitochondria, and lipidosome. Moreover, transcriptome and proteome analysis indicated that MTE-1 inhibited the growth of by inhibiting the synthesis of lipids, altering the primary metabolic pathways including carbohydrates and amino acid metabolism, and affecting the intracellular redox dyshomeostasis, thus leading to the disorder of active oxygen metabolism. These findings lay the foundation for the future application of MTE-1-derived agents in the management of antifungal diseases.
是一种致病真菌，通过花丝在水稻开花期引起假黑穗病。目前，需要开发安全有效的抗真菌剂来控制这种疾病。在我们的初步实验中，我们发现新的三糖酯 MTE-1 对具有显著的抑制活性。因此，研究了 MTE-1 在 中的作用和抑制机制。结果表明，MTE-1 以 5.67μg/mL 的 IC 抑制 菌丝生长。同样，MTE-1 破坏了 中的内膜系统，特别是质膜、线粒体和脂体。此外，转录组和蛋白质组分析表明，MTE-1 通过抑制脂质合成、改变碳水化合物和氨基酸代谢等主要代谢途径以及影响细胞内氧化还原失衡来抑制 的生长，从而导致活性氧代谢紊乱。这些发现为未来利用 MTE-1 衍生剂管理抗真菌病奠定了基础。
