IMPORTANCE: The Activity Card Sort is a valid, widely used measure of participation. There is a need for remotely delivered measures of participation to support the growing use of telehealth. OBJECTIVE: To develop and test the concurrent validity and acceptability of the electronic Activity Card Sort (ACS3). DESIGN: Cross-sectional. SETTING: Research laboratory. PARTICIPANTS: Community-dwelling adults. OUTCOMES AND MEASURES: The Activity Card Sort (ACS) and the ACS3 were administered in randomized order. Relationships between performance on the overlapping items of the ACS and ACS3 were evaluated using Spearman's ρ correlations. Additionally, acceptability of the ACS3 was evaluated using a survey with a 5-point Likert scale. RESULTS: High correlations were found between each of the four domains (rs ≥ .836) and total current activities score (rs = .863) between the ACS and ACS3. Ratings on the survey indicate high levels of acceptability and usability for the ACS3. CONCLUSIONS AND RELEVANCE: Findings suggest that performance on the ACS3 is consistent with performance on the ACS. This finding coupled with a high level of acceptability indicates that the ACS3 may be a clinically useful tool for evaluating daily life participation. What This Article Adds: The ACS3 provides an electronic, paperless option for measuring multiple domains of participation in adults.