Department of Medicine, Renal Division, Hospital 1, Peking University, Beijing, 100034, PR China; School of Materials Science and Engineering, Peking University, Beijing, 100871, PR China.
School of Materials Science and Engineering, Peking University, Beijing, 100871, PR China.
Talanta. 2022 Oct 1;248:123592. doi: 10.1016/j.talanta.2022.123592. Epub 2022 May 27.
In clinical practice, sera creatinine level is regarded as a crucial biomarker for the diagnosis, staging and monitoring of kidney disease. An amperometric biosensor is rapid, accurate, and cost-effective, with a portability and a simple operation. Herein, we report for the firsttime a disposable, printed amperometric biosensor for the clinical evaluation of creatinine in renal function detection. The sensor is constructed based on Prussian blue/carbon-graphite paste as the working electrode and the immobilization of creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase. The creatinine biosensor shows a linear detection range from 0.05 to 1.4 mM with a detection time of about 3 min. In addition, the sensor shows a high stability that can maintain above 86% of the initial activity after being stored for over 4 months. Moreover, the sensor shows almost the same results as those with the Jaffe method for measuring the real blood samples. We anticipate that the creatinine biosensor could be widely used in the medical and healthcare areas, especially for at-home testing and onsite medical examinations.
在临床实践中，血清肌酐水平被视为诊断、分期和监测肾脏疾病的重要生物标志物。电流型生物传感器快速、准确、经济高效，具有便携性和简单的操作。在此，我们首次报道了一种用于临床评估肾功能检测中肌酐的一次性、印刷式电流型生物传感器。该传感器基于普鲁士蓝/碳糊作为工作电极，并固定肌酐 amidohydrolase、creatine amidinohydrolase 和 sarcosine oxidase。肌酐生物传感器的线性检测范围为 0.05 至 1.4 mM，检测时间约为 3 分钟。此外，该传感器具有较高的稳定性，储存超过 4 个月后仍能保持初始活性的 86%以上。此外，该传感器在测量实际血样时与 Jaffe 法的结果几乎相同。我们预计肌酐生物传感器将在医疗保健领域得到广泛应用，特别是在家庭测试和现场医疗检查中。
