Healthsens S.L, Vivarium Ciencias de la Salud, calle Colegio Santo Domingo de Guzmán s/n, 33011 Oviedo, Spain.
Healthsens S.L, Vivarium Ciencias de la Salud, calle Colegio Santo Domingo de Guzmán s/n, 33011 Oviedo, Spain; Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain.
Clin Chim Acta. 2023 Mar 15;543:117300. doi: 10.1016/j.cca.2023.117300. Epub 2023 Mar 21.
An electrochemical biosensor for creatinine determination in a drop of whole human blood was developed and applied to the determination of creatinine in real clinical samples. It is based on the modification of a dual carbon working electrode with a combination of three enzymes: creatinine amidohydrolase (CNN), creatine amidinohydrolase (CRN) and sarcosine oxidase (SOX). Electrochemical transduction is performed using horseradish peroxidase (HRP) and potassium hexacyanoferrate(II) as mediator. A drop of human blood is enough to carry out the measurements by differential chronoamperometry where one carbon electrode detects creatine and the other both creatine and creatinine. The integrated differential signal obtained in the biosensor is linear with the concentration of creatinine in blood in the range 0.5-15 mg/dL and the enzyme-modified electrodes are stable for at least 3 months at 4 °C. The biosensor was lined to a reference method based on Isotope Dilution Mass Spectrometry (IDMS) with 50 real human blood samples and the results compared with those obtained by alternative routine techniques based on Jaffé method and an enzymatic method (Cobas 8000 Roche®, Crep2 Roche®). There were no significant differences between the creatinine concentrations found by the routine techniques and the developed biosensor.
研制了一种用于在全血样本中检测肌酸酐的电化学生物传感器，并将其应用于实际临床样本中肌酸酐的检测。该传感器基于双碳工作电极的修饰，修饰电极结合了三种酶：肌酸酰胺水解酶（CNN）、肌氨酸 amidinohydrolase（CRN）和肌氨酸氧化酶（SOX）。电化学转导采用辣根过氧化物酶（HRP）和亚铁氰化钾（II）作为介体进行。只需一滴全血即可通过差分计时安培法进行测量，其中一个碳电极检测肌酸，另一个电极同时检测肌酸和肌酸酐。在生物传感器中获得的集成差分信号与血液中肌酸酐的浓度呈线性关系，范围为 0.5-15mg/dL，并且酶修饰电极在 4°C 下至少稳定 3 个月。该生物传感器与基于同位素稀释质谱法（IDMS）的参考方法相连接，对 50 份真实人体血液样本进行了比较，结果与基于 Jaffé 法和酶法（罗氏 Cobas 8000，Crep2 Roche®）的替代常规技术的结果进行了比较。常规技术和开发的生物传感器检测到的肌酸酐浓度之间没有显著差异。
