Department of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan, 46241, Republic of Korea.
Department of Polymer Science and Engineering, Pusan National University, Busan, 46241, Republic of Korea.
Anal Chim Acta. 2021 Aug 29;1175:338749. doi: 10.1016/j.aca.2021.338749. Epub 2021 Jun 9.
A new disposable amperometric biosensor for sarcosine (Sar, a biomarker for prostate cancer) was designed based on screen-printed carbon electrodes, Prussian blue, polymer dispersed reduced graphene oxide (P-rGO) nanosheets, and sarcosine oxidase (SOx). Poly(sodium 4-styrenesulfonate-r-LAHEMA) denoted as PSSL was newly synthesized as dispersant for rGO. The P-rGO was utilized for SOx immobilization, the sulfonate and disulfide functionalities in PSSL enable physical adsorption of SOx and its bioactivity and stability properties were improved. The biosensor was optimized by various enzyme concentration, applied potential, and operating pH. Under the optimized conditions, the biosensor exhibited maximum current responses within 5 s at an applied potential of -0.1 V vs. integrated Ag/AgCl reference electrode. The biosensor had a dynamic linear range of 10-400 μM, with a sensitivity of 9.04 μA mM cm and a low detection limit of 0.66 μM (S/N = 3). Additionally, the biosensor possesses strong anti-interference capability, high reproducibility, and storage stability over 3 weeks. Furthermore, its clinical applicability was tested in urine samples from both prostate cancer patients and healthy control, and the analytical recoveries were satisfactory. Therefore, this biosensor has significant potential in the rapid and non-invasive point-of-care testing for prostate cancer diagnosis.
一种新的基于丝网印刷碳电极、普鲁士蓝、聚合物分散还原氧化石墨烯（P-rGO）纳米片和肌氨酸氧化酶（SOx）的肌氨酸（Sar，前列腺癌的生物标志物）一次性安培生物传感器被设计出来。新合成的聚（对苯乙烯磺酸钠-r-左旋乳酸）表示为 PSSL，用作 rGO 的分散剂。P-rGO 用于 SOx 的固定化，PSSL 中的磺酸盐和二硫键功能可以实现 SOx 的物理吸附，并提高其生物活性和稳定性。通过各种酶浓度、应用电位和操作 pH 值对生物传感器进行了优化。在优化条件下，生物传感器在-0.1 V 相对于集成 Ag/AgCl 参比电极的应用电位下，在 5 s 内显示出最大的电流响应。生物传感器具有 10-400 μM 的动态线性范围，灵敏度为 9.04 μA mM cm，检测限低至 0.66 μM（S/N = 3）。此外，该生物传感器具有较强的抗干扰能力、高重现性和超过 3 周的存储稳定性。此外，它在来自前列腺癌患者和健康对照的尿液样本中的临床适用性进行了测试，分析回收率令人满意。因此，该生物传感器在前列腺癌诊断的快速、非侵入性即时检测方面具有重要的应用潜力。
