School of Public Health, Nantong University, Nantong, 226019, Jiangsu Province, People's Republic of China.
Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
Mikrochim Acta. 2024 Aug 13;191(9):534. doi: 10.1007/s00604-024-06606-8.
Screen-printed carbon electrodes (SPCE) functionalized with MXene-based three-dimensional nanomaterials are reported for rapid determination of creatinine. TiCT MXene with in situ reduced AuNPs (MXene@AuNP) were used as a coreactant accelerator for efficient immobilization of enzymes. Creatinine could be oxidized by chitosan-embedded creatinine amidohydrolase, creatine amidinohydrolase, or sarcosine oxidase to generate HO, which could be electrochemically detected enhanced by Prussian blue (PB). The enzyme@CS/PB/MXene@AuNP/SPCE detected creatinine within the range 0.03-4.0 mM, with a limit of detection of 0.01 mM, with an average recovery of 96.8-103.7%. This indicates that the proposed biosensor is capable of detecting creatinine in a short amount of time (4 min) within a ± 5% percentage error, in contrast with the standard clinical colorimetric method. With this approach, reproducible and stable electrochemical responses could be achieved for determination of creatinine in serum, urine, or saliva. These results demonstrated its potential for deployment in resource-limited settings for early diagnosis and tracking the progression of chronic kidney disease (CKD).
基于 MXene 的三维纳米材料功能化的丝网印刷碳电极 (SPCE) 被用于快速测定肌酸酐。TiCT MXene 原位还原的 AuNPs (MXene@AuNP) 被用作核心反应物加速剂，以有效固定酶。壳聚糖嵌入的肌酸酰胺水解酶、肌氨酸 amidinohydrolase 或肌氨酸氧化酶可将肌酸氧化生成 HO，HO 可通过普鲁士蓝 (PB) 增强电化学检测。酶@CS/PB/MXene@AuNP/SPCE 在 0.03-4.0 mM 范围内检测肌酸酐，检出限为 0.01 mM，平均回收率为 96.8-103.7%。这表明，与标准临床比色法相比，该生物传感器能够在短时间（4 分钟）内以 ±5%的误差百分比检测到肌酸酐。通过这种方法，可以在血清、尿液或唾液中进行肌酸酐的测定，实现可重复和稳定的电化学响应。这些结果表明，它有可能在资源有限的环境中用于早期诊断和跟踪慢性肾病 (CKD) 的进展。
