Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture, Yangtze University, Jingzhou 434000, Hubei, China.
Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture, Yangtze University, Jingzhou 434000, Hubei, China; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Biol Macromol. 2022 Aug 31;215:262-271. doi: 10.1016/j.ijbiomac.2022.06.002. Epub 2022 Jun 4.
Drought stress has been the main abiotic factor affecting the growth, development and production of common buckwheat (Fagopyrum esculentum). To explore the response mechanisms of regulating buckwheat drought stress on the post-transcriptional and translational levels, a comparative proteomic analysis was applied to monitor the short-term proteomic variations under the drought stress in the seedling stage. From which 593 differentially abundant proteins (DAPs) were identified using the TMT-based proteomics analysis. A number of DAPs were found to be intimately correlated with the styrene degradation, phenylpropanoid biosynthesis and stimulus response, within which. The acyl-CoA oxidase 4 (ACX4), a key regulator in plant abiotic stress response, was selected for further elucidation. Overexpression of the FeACX4 not only conferred drought and salt tolerance in the Arabidopsis, but also significantly increased the root length and fresh weight in the overexpression lines plant relative to the wild type (WT) plant, accompanied by the elevated activities of catalase (CAT) and lowered malonaldehyde (MDA) and HO contents, therefore allowing plants to better adapt to adverse environments. Our results provided information in the exploring of the molecular regulation mechanism responding to drought tolerance in common buckwheat.
干旱胁迫一直是影响荞麦（Fagopyrum esculentum）生长、发育和产量的主要非生物因素。为了探索调节荞麦干旱胁迫的转录后和翻译水平的响应机制，采用比较蛋白质组学分析方法监测了苗期干旱胁迫下的短期蛋白质组变化。利用基于 TMT 的蛋白质组学分析，共鉴定出 593 个差异丰度蛋白（DAP）。发现许多 DAP 与苯乙烯降解、苯丙素生物合成和刺激反应密切相关，其中。酰基辅酶 A 氧化酶 4（ACX4）是植物非生物胁迫反应的关键调节剂，被选为进一步阐明的对象。与野生型（WT）植物相比，过表达 FeACX4 不仅赋予拟南芥耐旱耐盐性，而且还显著增加了过表达系植物的根长和鲜重，同时伴随着过氧化氢酶（CAT）活性的提高和丙二醛（MDA）和羟基含量的降低，从而使植物能够更好地适应不利环境。我们的研究结果为探索荞麦响应耐旱性的分子调控机制提供了信息。
