Hotchkin P G, Fallon A M
Biochim Biophys Acta. 1987 May 19;924(2):352-9. doi: 10.1016/0304-4165(87)90033-x.
Ribosome accumulation and synthesis in the fat body of the mosquito Aedes aegypti increased by approx. 4-fold during 18 h after the blood meal, consistent with the pattern of total RNA accumulation during the synthetic phase of the vitellogenic cycle. By 24-30 h after the blood meal, the accumulated ribosomes began to be degraded, and the total RNA content in the fat body eventually returned to previtellogenic levels. A method has been developed for isolation of ribosomal subunits from fat body, and the ribosomal proteins have been shown to have properties similar to those from cultured Aedes albopictus cells by two-dimensional polyacrylamide gel electrophoresis. Proteins S1, S10, and A from the small ribosomal subunit are phosphorylated when fat body is incubated in Aedes saline in the presence of [32P]orthophosphate.
在埃及伊蚊的脂肪体中，核糖体的积累和合成在血餐后18小时内增加了约4倍，这与卵黄发生周期合成阶段总RNA积累的模式一致。血餐后24至30小时，积累的核糖体开始降解，脂肪体中的总RNA含量最终恢复到卵黄发生前的水平。已经开发出一种从脂肪体中分离核糖体亚基的方法，并且通过二维聚丙烯酰胺凝胶电泳显示核糖体蛋白具有与来自白纹伊蚊培养细胞的核糖体蛋白相似的特性。当脂肪体在含有[32P]正磷酸盐的伊蚊盐溶液中孵育时，小核糖体亚基中的蛋白质S1、S10和A会被磷酸化。
