Lopez April D, Debnath Tathagata, Pinch Matthew, Hansen Immo A
New Mexico State University, Las Cruces, NM, 88003, USA.
The University of Texas at El Paso, El Paso, TX, 79968, USA.
Heliyon. 2024 Nov 12;10(22):e40060. doi: 10.1016/j.heliyon.2024.e40060. eCollection 2024 Nov 30.
The mosquito fat body is the principal source of yolk protein precursors (YPP) during mosquito egg development in female . To better understand the metabolic and signaling pathways involved in mosquito reproduction, we investigated changes in the mosquito fat body phosphoproteome at multiple time points after a blood meal. Using LC/MS, we identified 3570 phosphorylated proteins containing 14,551 individual phosphorylation sites. We observed protein phosphorylation changes in cellular pathways required for vitellogenesis, as well as proteins involved in primary cellular functions. Specifically, after a blood meal, proteins involved in ribosome synthesis, transcription, translation, and autophagy showed dynamic changes in their phosphorylation patterns. Our results provide new insight into blood meal-induced fat body dynamics and reveal potential proteins that can be targeted for interference with mosquito reproduction. Considering the devastating impact of mosquitoes on human health, worldwide, new approaches to control mosquitoes are urgently needed.
在雌性蚊子的卵发育过程中，蚊脂肪体是卵黄蛋白前体（YPP）的主要来源。为了更好地理解参与蚊子繁殖的代谢和信号通路，我们研究了血餐后多个时间点蚊子脂肪体磷酸化蛋白质组的变化。使用液相色谱/质谱联用技术，我们鉴定出3570种磷酸化蛋白质，包含14551个单独的磷酸化位点。我们观察到在卵黄生成所需的细胞途径以及参与主要细胞功能的蛋白质中存在蛋白质磷酸化变化。具体而言，血餐后，参与核糖体合成、转录、翻译和自噬的蛋白质在其磷酸化模式上表现出动态变化。我们的结果为血餐诱导的脂肪体动态变化提供了新的见解，并揭示了可作为干扰蚊子繁殖靶点的潜在蛋白质。考虑到蚊子对全球人类健康的毁灭性影响，迫切需要新的蚊子控制方法。
