MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China.
State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, 510641, China.
Nat Commun. 2022 Jun 7;13(1):3149. doi: 10.1038/s41467-022-30713-w.
Phototheranostics based on upconversion nanoparticles (UCNPs) offer the integration of imaging diagnostics and phototherapeutics. However, the programmable control of the photoactivation of imaging and therapy with minimum side effects is challenging due to the lack of ideal switchable UCNPs agents. Here we demonstrate a facile strategy to switch the near infrared emission at 800 nm from rationally designed UCNPs by modulating the irradiation laser into pulse output. We further synthesize a theranostic nanoagent by combining with a photosensitizer and a photoabsorbing agent assembled on the UCNPs. The orthogonal activation of in vivo photoacoustic imaging and photodynamic therapy can be achieved by altering the excitation modes from pulse to continuous-wave output upon a single 980 nm laser. No obvious harmful effects during photoexcitation was identified, suggesting their use for long-term imaging-guidance and phototherapy. This work provides an approach to the orthogonal activation of imaging diagnostics and photodynamic therapeutics.
基于上转换纳米粒子（UCNPs）的光热治疗将成像诊断和光疗结合在一起。然而，由于缺乏理想的可切换 UCNPs 试剂，因此具有最小副作用的成像和治疗的光激活的可编程控制具有挑战性。在这里，我们通过将照射激光调制为脉冲输出，展示了一种通过合理设计的 UCNPs 切换近红外发射（800nm）的简便策略。我们进一步通过将组装在 UCNPs 上的光敏剂和光吸收剂结合来合成治疗纳米剂。通过将激发模式从脉冲切换到连续波输出，可以在单个 980nm 激光下实现体内光声成像和光动力治疗的正交激活。在光激发过程中未发现明显的有害影响，这表明它们可用于长期成像指导和光疗。这项工作为成像诊断和光动力治疗的正交激活提供了一种方法。
