Department of Genetics-Plant Breeding and Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Himachal Pradesh, 173101, India.
Government Model College, Jhabua, Madhya Pradesh, 457661, India.
Mol Biotechnol. 2022 Nov;64(11):1198-1217. doi: 10.1007/s12033-022-00507-y. Epub 2022 Jun 7.
Plant gene editing has become an important molecular tool to revolutionize modern breeding of crops. Over the past years, remarkable advancement has been made in developing robust and efficient editing methods for plants. Despite a variety of available genome editing methods, the discovery of most recent system of clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) has been one of the biggest advancement in this path, with being the most efficient approach for genome manipulation. Until recently, genetic manipulations were confined to methods, like Agrobacterium-mediated transformations, zinc-finger nucleases, and TAL effector nucleases. However this technology supersedes all other methods for genetic modification. This RNA-guided CRISPR-Cas system is being rapidly developed with enhanced functionalities for better use and greater possibilities in biological research. In this review, we discuss and sum up the application of this simple yet powerful tool of CRISPR-Cas system for crop improvement with recent advancement in this technology.
植物基因编辑已成为革新作物现代育种的重要分子工具。在过去的几年中，开发用于植物的强大且高效的编辑方法方面取得了显著进展。尽管有多种可用的基因组编辑方法，但最近发现的成簇规律间隔短回文重复序列 - CRISPR 相关蛋白（CRISPR-Cas）系统是这一领域的最大进展之一，是基因组操作最有效的方法。直到最近，遗传操作还局限于方法，如农杆菌介导的转化、锌指核酸酶和 TAL 效应物核酸酶。然而，这项技术已经取代了其他所有的遗传修饰方法。这种 RNA 引导的 CRISPR-Cas 系统正在快速发展，具有增强的功能，以便在生物研究中更好地使用和提供更大的可能性。在这篇综述中，我们讨论并总结了 CRISPR-Cas 系统这一简单而强大的工具在作物改良中的应用，并介绍了该技术的最新进展。
