Mazumdar Suchismita, Marar Thankamani, Patki Jyoti
School of Biotechnology and Bioinformatics, D.Y. Patil Deemed to be University, Sector-15, Plot-50, CBD Belapur, Navi Mumbai, 400614 India.
J Diabetes Metab Disord. 2022 Jan 20;21(1):323-332. doi: 10.1007/s40200-022-00977-6. eCollection 2022 Jun.
The present study was aimed at analysing the reno-protective potential of Gedunin (a limonoid from Neem leaves) through haematological and serological assays and renal histopathology studies in streptozotocin induced diabetic rats.
Four weeks old Wister albino rats were chosen for the study. The rats were divided into four groups of 8 rats each. Group 1 (normal control rats); Group 2 (diabetic control rats); Group 3 (Drug control rats: normal rats treated with 1 mg/kg body weight of Gedunin thrice a week through oral gavage for 2 weeks); Group 4 (diabetic rats treated with Gedunin similar to Group 3). Diabetes was induced using a single intraperitoneal injection of streptozotocin (50 mg/kg) in the pre-determined groups. After completion of treatment, the rats were sacrificed and haematological and serological parameters including functional indices of blood cells and renal markers were evaluated through spectrophotometric methods. Renal histology studies were carried out and images were captured on Olympus SC 100, 10 Mega Pixel camera.
Streptozotocin induced diabetes lead to marked reduction in the RBC count, haemoglobin, PCV, WBC count, lymphocyte, elevated levels of fasting blood glucose, kidney function markers such as urea, creatinine, uric acid in rats. Administration of Gedunin significantly ( < 0.05) ameliorated the elevated levels of fasting glucose, RDW%, MCHC%, NLR% and platelet count. A significant reversal of renal tissue damage was also observed.
The restoration of renal serological markers and reversal of renal tissue injuries are all indicative of promising reno-protective potential of Gedunin. Remarkable improvement of haematological parameters supports their use as reno-protection markers.
本研究旨在通过血液学和血清学检测以及对链脲佐菌素诱导的糖尿病大鼠进行肾脏组织病理学研究，分析格杜宁（一种从印楝叶中提取的柠檬苦素）的肾脏保护潜力。
选取4周龄的Wistar白化大鼠进行研究。将大鼠分为四组，每组8只。第1组（正常对照大鼠）；第2组（糖尿病对照大鼠）；第3组（药物对照大鼠：正常大鼠每周经口灌胃3次，每次给予1mg/kg体重的格杜宁，持续2周）；第4组（与第3组相同方式用格杜宁治疗的糖尿病大鼠）。在预定组中通过单次腹腔注射链脲佐菌素（50mg/kg）诱导糖尿病。治疗结束后，处死大鼠，通过分光光度法评估血液学和血清学参数，包括血细胞功能指标和肾脏标志物。进行肾脏组织学研究，并使用奥林巴斯SC 100 1000万像素相机拍摄图像。
链脲佐菌素诱导的糖尿病导致大鼠红细胞计数、血红蛋白、红细胞压积、白细胞计数、淋巴细胞显著减少，空腹血糖、肾功能标志物如尿素、肌酐、尿酸水平升高。给予格杜宁可显著（<0.05）改善空腹血糖、红细胞分布宽度百分比、平均血红蛋白浓度百分比、中性粒细胞与淋巴细胞比值百分比和血小板计数的升高水平。还观察到肾脏组织损伤有显著逆转。
肾脏血清学标志物的恢复和肾脏组织损伤的逆转均表明格杜宁具有良好的肾脏保护潜力。血液学参数的显著改善支持将其用作肾脏保护标志物。
