Department of Rheumatology and Immunology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, P. R. China.
Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Material Sciences and Chemical Engineering, Harbin Engineering University, Harbin 150001, P. R. China.
J Mater Chem B. 2022 Jun 22;10(24):4662-4671. doi: 10.1039/d1tb02657g.
Fluorescence imaging with advanced multi-channel observation, high sensitivity, and high spatio-temporal resolution holds great promise for biomedical applications. However, the different absorption and scattering spectra of complex tissue structures present significant challenges for real-time investigations of live biological and physiological processes. Herein, we present Yb/Er/Ho-engineered rare earth fluoride nanoparticles with integrated multimodal imaging functions that can provide synergistic effects over any modality alone. The engineered nanoparticles can realize efficient and accurate diagnoses in clinical research. Employing Yb sensitizers and both Er and Ho emitters, NaGdF:15%Yb,15%Er,%Ho nanoparticles absorb an excitation wavelength of 980 nm and emit luminescence centered at 1525 nm and 1155 nm in the second near-infrared (NIR-II) window. In addition, upconversion emission in the visible region is also observed for multiplex signals. - and -weighted magnetic resonance (MR) imaging is achieved owing to the presence of paramagnetic Gd and Ho species. The high X-ray attenuation ability of the elemental constituents permits the use of the prepared nanoparticles as high-contrast X-ray computed tomography (CT) imaging agents. Taken together, this study shows the construction of an alternative multimodal nanoprobe with synergistic - and -MR/CT/downconversion luminescence (DCL) imaging abilities, which can provide an alternative approach for disease diagnosis and supervision.
基于先进的多通道观测、高灵敏度和高时空分辨率的荧光成像是生物医学应用的一个重要发展方向。然而，复杂组织结构的不同吸收和散射光谱对实时研究活的生物和生理过程带来了重大挑战。在此，我们提出了具有集成多模态成像功能的 Yb/Er/Ho 设计的稀土氟化物纳米粒子，这些功能可以提供任何单一模态所无法实现的协同效应。这些设计的纳米粒子可以在临床研究中实现高效和准确的诊断。采用 Yb 敏化剂和 Er 和 Ho 两种发光体，NaGdF4:15%Yb,15%Er,%Ho 纳米粒子吸收 980nm 的激发波长，并在第二近红外（NIR-II）窗口中发射出中心波长为 1525nm 和 1155nm 的荧光。此外，还观察到了可见区域的上转换发射，实现了多重信号。由于存在顺磁的 Gd 和 Ho 物种，实现了磁共振（MR）成像的 T1 和 T2 加权。元素成分的高 X 射线衰减能力使得所制备的纳米粒子可用作高对比度 X 射线计算机断层扫描（CT）成像剂。总之，本研究展示了一种替代的多模态纳米探针的构建，具有协同的 MR/CT/下转换发光（DCL）成像能力，为疾病诊断和监测提供了一种替代方法。
