Weng Ye, Zhang Hongyan, Xu Shujun, Zhao Yue, Ma Lisha, Shi Jingbin, Qian Ke, Liang Weizong, Xiong Yang
The Fourth School of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou 310053, China.
School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, China.
Curr Drug Deliv. 2023;20(2):183-191. doi: 10.2174/1567201819666220607153457.
To improve the solubility of Honokiol (HNK), Honokiol nanoparticles (HNK-NPs) were prepared using a new biodegradable polysaccharide polymer as its carrier.
HNK-NPs were prepared by hydrophilic polymer coagulation method, and the processing parameters were optimized according to average particle size and PDI by a single factor experiment. The morphology of the optimized nanoparticles was investigated by TEM, and the in vitro release was carried out to evaluate the optimized HNK-NPs.
The encapsulation efficiency and drug loading of the HNK-NPs were 77.75 ± 2.63% and 13.46 ± 0.39%, respectively. The obtained nanoparticles of HNK-NPs were spherical-like under the electron microscope with a mean particle size of 198.50 ± 0.01 nm and a Zeta potential of -52.60 ± 1.00 mV. The in vitro release results showed that the cumulative release rates of nanoparticles were 48.28 ± 9.80% and 81.12 ± 4.35% within 2 h and 8 h, respectively, showing a stable release behavior. The average particle size and PDI of HNK-NPs solution prepared by the hydrophilic polymer condensation method had no obvious change at 72h.
HNK-NPs were successfully prepared by the phase separation method. This new polysaccharide polymer should be an ideal carrier to help improve the solubility of HNK.
为提高厚朴酚（HNK）的溶解度，以一种新型可生物降解的多糖聚合物为载体制备厚朴酚纳米粒（HNK-NPs）。
采用亲水性聚合物凝聚法制备HNK-NPs，并通过单因素实验根据平均粒径和PDI对工艺参数进行优化。通过透射电子显微镜（TEM）研究优化后纳米粒的形态，并进行体外释放实验以评估优化后的HNK-NPs。
HNK-NPs的包封率和载药量分别为77.75±2.63%和13.46±0.39%。在电子显微镜下观察到所得的HNK-NPs纳米粒呈球形，平均粒径为198.50±0.01nm，Zeta电位为-52.60±1.00mV。体外释放结果表明，纳米粒在2小时和8小时内的累积释放率分别为48.28±9.80%和81.12±4.35%，呈现出稳定的释放行为。通过亲水性聚合物凝聚法制备的HNK-NPs溶液在72小时时平均粒径和PDI无明显变化。
通过相分离法成功制备了HNK-NPs。这种新型多糖聚合物应是有助于提高HNK溶解度的理想载体。
