Quaid-i-Azam University, Faculty of Biological Science, Department of Plant Sciences, Islamabad, Pakistan.
Quaid-i-Azam University, National Centre for Physics, Islamabad, Pakistan.
Braz J Biol. 2022 Jun 1;84:e261123. doi: 10.1590/1519-6984.261123. eCollection 2022.
Nature is gifted with a wide range of ornamental plants, which beautify and clean the nature. Due to its great aesthetic value, there is a need to protect these plants from a variety of biotic and abiotic stresses. Hibiscus rosa-sinensis (L.) is an ornamental plant and it is commonly known as China rose or shoeblack plant. It is affected by several fungal and bacterial pathogens. Current study was designed to isolate leaf spot pathogen of H. rosa-sinensis and its control using silver nanoparticles (AgNPs). Based on molecular and morphological features, the isolated leaf spot pathogen was identified as Aspergillus niger. AgNPs were synthesized in the leaf extract of Calotropis procera and characterized. UV-vis spectral analysis displayed discrete plasmon resonance bands on the surface of synthesized AgNPs, depicting the presence of aromatic amino acids. Fourier transform infrared spectroscopy (FTIR) described the presence of C-O, NH, C-H, and O-H functional groups, which act as stabilizing and reducing molecules. X-ray diffraction (XRD) revealed the average size (~32.43 nm) of AgNPs and scanning electron microscopy (SEM) depicted their spherical nature. In this study, in vitro and in vivo antifungal activity of AgNPs was investigated. In vitro antifungal activity analysis revealed the highest growth inhibition of mycelia (87%) at 1.0 mg/ml concentration of AgNPs. The same concentration of AgNPs tremendously inhibited the spread of disease on infected leaves of H. rosa-sinensis. These results demonstrated significant disease control ability of AgNPs and suggested their use on different ornamental plants.
大自然拥有广泛的观赏植物，这些植物美化和净化了大自然。由于其具有巨大的美学价值，因此需要保护这些植物免受各种生物和非生物胁迫的影响。木槿（Hibiscus rosa-sinensis（L.））是一种观赏植物，通常被称为中国玫瑰或鞋刷植物。它受到几种真菌和细菌病原体的影响。本研究旨在分离木槿叶斑病的病原体，并使用银纳米粒子（AgNPs）进行控制。根据分子和形态特征，分离出的叶斑病病原体被鉴定为黑曲霉（Aspergillus niger）。AgNPs 是在 Calotropis procera 的叶提取物中合成的，并进行了表征。紫外可见光谱分析显示在合成的 AgNPs 表面上存在离散的等离子体共振带，表明存在芳香族氨基酸。傅立叶变换红外光谱（FTIR）描述了存在 C-O、NH、C-H 和 O-H 官能团，它们作为稳定和还原分子。X 射线衍射（XRD）揭示了 AgNPs 的平均尺寸（约 32.43nm），扫描电子显微镜（SEM）描绘了它们的球形性质。在这项研究中，研究了 AgNPs 的体外和体内抗真菌活性。体外抗真菌活性分析表明，AgNPs 的浓度为 1.0mg/ml 时对菌丝体的生长抑制率最高（87%）。相同浓度的 AgNPs 极大地抑制了感染木槿叶片上疾病的扩散。这些结果表明 AgNPs 具有显著的疾病控制能力，并表明它们可用于不同的观赏植物。
